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Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers.

Publication ,  Journal Article
Quiroz, FG; Chilkoti, A
Published in: Nature materials
November 2015

Proteins and synthetic polymers that undergo aqueous phase transitions mediate self-assembly in nature and in man-made material systems. Yet little is known about how the phase behaviour of a protein is encoded in its amino acid sequence. Here, by synthesizing intrinsically disordered, repeat proteins to test motifs that we hypothesized would encode phase behaviour, we show that the proteins can be designed to exhibit tunable lower or upper critical solution temperature (LCST and UCST, respectively) transitions in physiological solutions. We also show that mutation of key residues at the repeat level abolishes phase behaviour or encodes an orthogonal transition. Furthermore, we provide heuristics to identify, at the proteome level, proteins that might exhibit phase behaviour and to design novel protein polymers consisting of biologically active peptide repeats that exhibit LCST or UCST transitions. These findings set the foundation for the prediction and encoding of phase behaviour at the sequence level.

Duke Scholars

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Published In

Nature materials

DOI

EISSN

1476-4660

ISSN

1476-1122

Publication Date

November 2015

Volume

14

Issue

11

Start / End Page

1164 / 1171

Related Subject Headings

  • Sequence Analysis, Protein
  • Repetitive Sequences, Amino Acid
  • Proteome
  • Nanoscience & Nanotechnology
  • Humans
  • Animals
 

Citation

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Quiroz, F. G., & Chilkoti, A. (2015). Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers. Nature Materials, 14(11), 1164–1171. https://doi.org/10.1038/nmat4418
Quiroz, Felipe García, and Ashutosh Chilkoti. “Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers.Nature Materials 14, no. 11 (November 2015): 1164–71. https://doi.org/10.1038/nmat4418.
Quiroz FG, Chilkoti A. Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers. Nature materials. 2015 Nov;14(11):1164–71.
Quiroz, Felipe García, and Ashutosh Chilkoti. “Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers.Nature Materials, vol. 14, no. 11, Nov. 2015, pp. 1164–71. Epmc, doi:10.1038/nmat4418.
Quiroz FG, Chilkoti A. Sequence heuristics to encode phase behaviour in intrinsically disordered protein polymers. Nature materials. 2015 Nov;14(11):1164–1171.

Published In

Nature materials

DOI

EISSN

1476-4660

ISSN

1476-1122

Publication Date

November 2015

Volume

14

Issue

11

Start / End Page

1164 / 1171

Related Subject Headings

  • Sequence Analysis, Protein
  • Repetitive Sequences, Amino Acid
  • Proteome
  • Nanoscience & Nanotechnology
  • Humans
  • Animals