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Enabling functional genomics with genome engineering.

Publication ,  Journal Article
Hilton, IB; Gersbach, CA
Published in: Genome research
October 2015

Advances in genome engineering technologies have made the precise control over genome sequence and regulation possible across a variety of disciplines. These tools can expand our understanding of fundamental biological processes and create new opportunities for therapeutic designs. The rapid evolution of these methods has also catalyzed a new era of genomics that includes multiple approaches to functionally characterize and manipulate the regulation of genomic information. Here, we review the recent advances of the most widely adopted genome engineering platforms and their application to functional genomics. This includes engineered zinc finger proteins, TALEs/TALENs, and the CRISPR/Cas9 system as nucleases for genome editing, transcription factors for epigenome editing, and other emerging applications. We also present current and potential future applications of these tools, as well as their current limitations and areas for future advances.

Duke Scholars

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Published In

Genome research

DOI

EISSN

1549-5469

ISSN

1088-9051

Publication Date

October 2015

Volume

25

Issue

10

Start / End Page

1442 / 1455

Related Subject Headings

  • Zinc Fingers
  • Transcriptional Activation
  • Humans
  • Genomics
  • Genetic Techniques
  • Genetic Engineering
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Bioinformatics
  • Animals
  • 3105 Genetics
 

Citation

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Hilton, I. B., & Gersbach, C. A. (2015). Enabling functional genomics with genome engineering. Genome Research, 25(10), 1442–1455. https://doi.org/10.1101/gr.190124.115
Hilton, Isaac B., and Charles A. Gersbach. “Enabling functional genomics with genome engineering.Genome Research 25, no. 10 (October 2015): 1442–55. https://doi.org/10.1101/gr.190124.115.
Hilton IB, Gersbach CA. Enabling functional genomics with genome engineering. Genome research. 2015 Oct;25(10):1442–55.
Hilton, Isaac B., and Charles A. Gersbach. “Enabling functional genomics with genome engineering.Genome Research, vol. 25, no. 10, Oct. 2015, pp. 1442–55. Epmc, doi:10.1101/gr.190124.115.
Hilton IB, Gersbach CA. Enabling functional genomics with genome engineering. Genome research. 2015 Oct;25(10):1442–1455.

Published In

Genome research

DOI

EISSN

1549-5469

ISSN

1088-9051

Publication Date

October 2015

Volume

25

Issue

10

Start / End Page

1442 / 1455

Related Subject Headings

  • Zinc Fingers
  • Transcriptional Activation
  • Humans
  • Genomics
  • Genetic Techniques
  • Genetic Engineering
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Bioinformatics
  • Animals
  • 3105 Genetics