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Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum.

Publication ,  Journal Article
Butt, CM; Miranda, ML; Stapleton, HM
Published in: Analytical and bioanalytical chemistry
April 2016

Polybrominated diphenyl ethers (PBDEs) flame retardants (FRs) were phased-out in the mid-2000s (penta- and octaBDE) and 2013 (decaBDE); however, their hydroxylated metabolites (OH-BDEs) are still commonly detected in human serum. Today, novel FRs such as Firemaster® 550, a mixture that contains two brominated compounds, EH-TBB and BEH-TEBP are used as replacements for PBDEs in some applications, and there is a need to develop a comprehensive analytical method to assess exposure to both legacy PBDEs and novel FRs. This study developed a solid-phase extraction (SPE)-based method to analyze PBDEs, OH-BDEs, 2,4,6-tribromophenol (TBP), hexabromocylcododecane isomers (HBCDs), EH-TBB, and BEH-TEBP in human serum. Briefly, serum proteins were first denatured with formic acid, and then the target analytes were isolated using a SPE column. Finally, the extract was cleaned and fractioned using a silica SPE column. Method performance was assessed by spiking fetal bovine serum with 1-2 ng of the target analytes, and method accuracy was quantified by comparison to a serum Standard Reference Material (SRM). The developed method showed good recovery and accuracy for all target analytes with the exception of the very low and very high molecular weight PBDE congeners. Using this method, 43 serum samples collected from the Healthy Pregnancy, Healthy Baby Study (HPHB) cohort in Durham, NC, USA were analyzed for FRs. A novel finding was the ubiquitous detection of 2,4,6-TBP, at levels greater than the individual PBDE congeners. Furthermore, 2,4,6-TBP was positively correlated with PBDEs, suggesting that they may have a similar source of exposure, or that 2,4,6-TBP may result from metabolism of PBDEs in vivo.

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Published In

Analytical and bioanalytical chemistry

DOI

EISSN

1618-2650

ISSN

1618-2642

Publication Date

April 2016

Volume

408

Issue

10

Start / End Page

2449 / 2459

Related Subject Headings

  • Solid Phase Extraction
  • Reference Standards
  • Humans
  • Flame Retardants
  • Blood
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences
  • 09 Engineering
 

Citation

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Chicago
ICMJE
MLA
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Butt, C. M., Miranda, M. L., & Stapleton, H. M. (2016). Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum. Analytical and Bioanalytical Chemistry, 408(10), 2449–2459. https://doi.org/10.1007/s00216-016-9340-3
Butt, Craig M., Marie Lynn Miranda, and Heather M. Stapleton. “Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum.Analytical and Bioanalytical Chemistry 408, no. 10 (April 2016): 2449–59. https://doi.org/10.1007/s00216-016-9340-3.
Butt CM, Miranda ML, Stapleton HM. Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum. Analytical and bioanalytical chemistry. 2016 Apr;408(10):2449–59.
Butt, Craig M., et al. “Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum.Analytical and Bioanalytical Chemistry, vol. 408, no. 10, Apr. 2016, pp. 2449–59. Epmc, doi:10.1007/s00216-016-9340-3.
Butt CM, Miranda ML, Stapleton HM. Development of an analytical method to quantify PBDEs, OH-BDEs, HBCDs, 2,4,6-TBP, EH-TBB, and BEH-TEBP in human serum. Analytical and bioanalytical chemistry. 2016 Apr;408(10):2449–2459.
Journal cover image

Published In

Analytical and bioanalytical chemistry

DOI

EISSN

1618-2650

ISSN

1618-2642

Publication Date

April 2016

Volume

408

Issue

10

Start / End Page

2449 / 2459

Related Subject Headings

  • Solid Phase Extraction
  • Reference Standards
  • Humans
  • Flame Retardants
  • Blood
  • Analytical Chemistry
  • 40 Engineering
  • 34 Chemical sciences
  • 31 Biological sciences
  • 09 Engineering