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Novel intrathecal and subcutaneous catheter delivery systems in the mouse.

Publication ,  Journal Article
Oladosu, FA; Ciszek, BP; O'Buckley, SC; Nackley, AG
Published in: J Neurosci Methods
May 1, 2016

BACKGROUND: Catheter systems that permit targeted delivery of genes, molecules, ligands, and other agents represent an investigative tool critical to the development of clinically relevant animal models that facilitate the study of neurological health and disease. The development of new sustained catheter delivery systems to spinal and peripheral sites will reduce the need for repeated injections, while ensuring constant levels of drug in plasma and tissues. NEW METHOD: Here, we introduce two novel catheter delivery systems in the mouse: the O'Buckley intrathecal catheter system for sustained delivery to the spinal region and a subcutaneous bifurcated catheter system for sustained drug delivery to both hindpaws. RESULTS: The O'Buckley intrathecal catheter system consistently distributed Evans Blue throughout the spinal cord, with the greatest concentration at the thoracic region, and with an 85% surgery success rate. The subcutaneous catheter system consistently distributed Evans Blue to the hindlimbs, with a 100% surgery success rate. COMPARISON TO EXISTING METHOD: The O'Buckley intrathecal catheter system accomplishes sustained drug delivery to the spinal region, with a 2-fold increase in surgery success rate, as compared to the traditional method. Our subcutaneous bifurcated catheter system accomplishes sustained drug delivery to both hindpaws, eliminating the need for repeated intraplantar injections. CONCLUSIONS: We have developed catheter systems that improve upon traditional methods in order to achieve sustained localized drug delivery to spinal tissues and to hindpaw tissues surrounding peripheral sciatic nerve terminals. These methods have a broad reach, and can be used to enhance behavioral, physiologic and mechanistic studies in mice.

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Published In

J Neurosci Methods

DOI

EISSN

1872-678X

Publication Date

May 1, 2016

Volume

264

Start / End Page

119 / 128

Location

Netherlands

Related Subject Headings

  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice, 129 Strain
  • Mice
  • Male
  • Injections, Spinal
  • Infusions, Subcutaneous
  • Female
  • Extremities
  • Drug Delivery Systems
 

Citation

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ICMJE
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Oladosu, F. A., Ciszek, B. P., O’Buckley, S. C., & Nackley, A. G. (2016). Novel intrathecal and subcutaneous catheter delivery systems in the mouse. J Neurosci Methods, 264, 119–128. https://doi.org/10.1016/j.jneumeth.2016.03.006
Oladosu, Folabomi A., Brittney P. Ciszek, Sandra C. O’Buckley, and Andrea G. Nackley. “Novel intrathecal and subcutaneous catheter delivery systems in the mouse.J Neurosci Methods 264 (May 1, 2016): 119–28. https://doi.org/10.1016/j.jneumeth.2016.03.006.
Oladosu FA, Ciszek BP, O’Buckley SC, Nackley AG. Novel intrathecal and subcutaneous catheter delivery systems in the mouse. J Neurosci Methods. 2016 May 1;264:119–28.
Oladosu, Folabomi A., et al. “Novel intrathecal and subcutaneous catheter delivery systems in the mouse.J Neurosci Methods, vol. 264, May 2016, pp. 119–28. Pubmed, doi:10.1016/j.jneumeth.2016.03.006.
Oladosu FA, Ciszek BP, O’Buckley SC, Nackley AG. Novel intrathecal and subcutaneous catheter delivery systems in the mouse. J Neurosci Methods. 2016 May 1;264:119–128.
Journal cover image

Published In

J Neurosci Methods

DOI

EISSN

1872-678X

Publication Date

May 1, 2016

Volume

264

Start / End Page

119 / 128

Location

Netherlands

Related Subject Headings

  • Neurology & Neurosurgery
  • Mice, Inbred C57BL
  • Mice, 129 Strain
  • Mice
  • Male
  • Injections, Spinal
  • Infusions, Subcutaneous
  • Female
  • Extremities
  • Drug Delivery Systems