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Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.

Publication ,  Journal Article
Chang, C-C; Mao, M; Liu, Y; Wu, M; Vo-Dinh, T; Yuan, F
Published in: Cellular and molecular bioengineering
December 2016

Electrotransfection has been widely used as a versatile, non-viral method for gene delivery. However, electrotransfection efficiency (eTE) is still low and unstable, compared to viral methods. To understand potential mechanisms of the unstable eTE, we investigated effects of electrode materials on eTE and viability of mammalian cells. Data from the study showed that commonly used metal electrodes generated a significant amount of particles during application of pulsed electric field, which could cause precipitation of plasmid DNA from solutions, thereby reducing eTE. For aluminum electrodes, the particles were composed of aluminum hydroxide and/or aluminum oxide, and their median sizes were 300 to 400 nm after the buffer being pulsed 4 to 8 times at 400 V cm-1, 5 ms duration and 1 Hz frequency. The precipitation could be prevented by using carbon (graphite) electrodes in electrotransfection experiments. The use of carbon electrodes also increased cell viability. Taken together, the study suggested that electrodes made of inner materials were desirable for electrotransfection of cells in vitro.

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Published In

Cellular and molecular bioengineering

DOI

EISSN

1865-5033

ISSN

1865-5025

Publication Date

December 2016

Volume

9

Issue

4

Start / End Page

538 / 545

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering
 

Citation

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Chang, C.-C., Mao, M., Liu, Y., Wu, M., Vo-Dinh, T., & Yuan, F. (2016). Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes. Cellular and Molecular Bioengineering, 9(4), 538–545. https://doi.org/10.1007/s12195-016-0452-9
Chang, Chun-Chi, Mao Mao, Yang Liu, Mina Wu, Tuan Vo-Dinh, and Fan Yuan. “Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.Cellular and Molecular Bioengineering 9, no. 4 (December 2016): 538–45. https://doi.org/10.1007/s12195-016-0452-9.
Chang C-C, Mao M, Liu Y, Wu M, Vo-Dinh T, Yuan F. Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes. Cellular and molecular bioengineering. 2016 Dec;9(4):538–45.
Chang, Chun-Chi, et al. “Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes.Cellular and Molecular Bioengineering, vol. 9, no. 4, Dec. 2016, pp. 538–45. Epmc, doi:10.1007/s12195-016-0452-9.
Chang C-C, Mao M, Liu Y, Wu M, Vo-Dinh T, Yuan F. Improvement in Electrotransfection of Cells Using Carbon-Based Electrodes. Cellular and molecular bioengineering. 2016 Dec;9(4):538–545.
Journal cover image

Published In

Cellular and molecular bioengineering

DOI

EISSN

1865-5033

ISSN

1865-5025

Publication Date

December 2016

Volume

9

Issue

4

Start / End Page

538 / 545

Related Subject Headings

  • 4003 Biomedical engineering
  • 0903 Biomedical Engineering