Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.
Publication
, Journal Article
Simic, T; Pljesa-Ercegovac, M; Savic-Radojevic, A; Hadziahmetovic, M; Mimic-Oka, J
Published in: Amino Acids
June 2006
To identify kidney glutathione S-transferase (GST) isoenzyme, which does not bind to glutathione affinity column, biochemical characterization was performed by using an array of substrates and by measuring sensitivity to inhibitors. Immunological characterization was done by immunoblotting. Affinity flow-through GST exhibited activity towards 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole and cumene hydroperoxide, typical class alpha substrates and high sensitivity towards hematin, an alpha class inhibitor. It cross-reacted with antibodies against alpha class GST. Affinity flow-through GST in human kidney is an alpha class member.
Duke Scholars
Published In
Amino Acids
DOI
ISSN
0939-4451
Publication Date
June 2006
Volume
30
Issue
4
Start / End Page
495 / 498
Location
Austria
Related Subject Headings
- Triazines
- Sensitivity and Specificity
- Kidney
- Immunoblotting
- Humans
- Hemin
- Glutathione Transferase
- Glutathione
- Ethacrynic Acid
- Enzyme Inhibitors
Citation
APA
Chicago
ICMJE
MLA
NLM
Simic, T., Pljesa-Ercegovac, M., Savic-Radojevic, A., Hadziahmetovic, M., & Mimic-Oka, J. (2006). Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids, 30(4), 495–498. https://doi.org/10.1007/s00726-006-0329-7
Simic, T., M. Pljesa-Ercegovac, A. Savic-Radojevic, M. Hadziahmetovic, and J. Mimic-Oka. “Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.” Amino Acids 30, no. 4 (June 2006): 495–98. https://doi.org/10.1007/s00726-006-0329-7.
Simic T, Pljesa-Ercegovac M, Savic-Radojevic A, Hadziahmetovic M, Mimic-Oka J. Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids. 2006 Jun;30(4):495–8.
Simic, T., et al. “Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.” Amino Acids, vol. 30, no. 4, June 2006, pp. 495–98. Pubmed, doi:10.1007/s00726-006-0329-7.
Simic T, Pljesa-Ercegovac M, Savic-Radojevic A, Hadziahmetovic M, Mimic-Oka J. Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids. 2006 Jun;30(4):495–498.
Published In
Amino Acids
DOI
ISSN
0939-4451
Publication Date
June 2006
Volume
30
Issue
4
Start / End Page
495 / 498
Location
Austria
Related Subject Headings
- Triazines
- Sensitivity and Specificity
- Kidney
- Immunoblotting
- Humans
- Hemin
- Glutathione Transferase
- Glutathione
- Ethacrynic Acid
- Enzyme Inhibitors