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Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.

Publication ,  Journal Article
Simic, T; Pljesa-Ercegovac, M; Savic-Radojevic, A; Hadziahmetovic, M; Mimic-Oka, J
Published in: Amino Acids
June 2006

To identify kidney glutathione S-transferase (GST) isoenzyme, which does not bind to glutathione affinity column, biochemical characterization was performed by using an array of substrates and by measuring sensitivity to inhibitors. Immunological characterization was done by immunoblotting. Affinity flow-through GST exhibited activity towards 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole and cumene hydroperoxide, typical class alpha substrates and high sensitivity towards hematin, an alpha class inhibitor. It cross-reacted with antibodies against alpha class GST. Affinity flow-through GST in human kidney is an alpha class member.

Duke Scholars

Published In

Amino Acids

DOI

ISSN

0939-4451

Publication Date

June 2006

Volume

30

Issue

4

Start / End Page

495 / 498

Location

Austria

Related Subject Headings

  • Triazines
  • Sensitivity and Specificity
  • Kidney
  • Immunoblotting
  • Humans
  • Hemin
  • Glutathione Transferase
  • Glutathione
  • Ethacrynic Acid
  • Enzyme Inhibitors
 

Citation

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Simic, T., Pljesa-Ercegovac, M., Savic-Radojevic, A., Hadziahmetovic, M., & Mimic-Oka, J. (2006). Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids, 30(4), 495–498. https://doi.org/10.1007/s00726-006-0329-7
Simic, T., M. Pljesa-Ercegovac, A. Savic-Radojevic, M. Hadziahmetovic, and J. Mimic-Oka. “Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.Amino Acids 30, no. 4 (June 2006): 495–98. https://doi.org/10.1007/s00726-006-0329-7.
Simic T, Pljesa-Ercegovac M, Savic-Radojevic A, Hadziahmetovic M, Mimic-Oka J. Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids. 2006 Jun;30(4):495–8.
Simic, T., et al. “Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney.Amino Acids, vol. 30, no. 4, June 2006, pp. 495–98. Pubmed, doi:10.1007/s00726-006-0329-7.
Simic T, Pljesa-Ercegovac M, Savic-Radojevic A, Hadziahmetovic M, Mimic-Oka J. Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Amino Acids. 2006 Jun;30(4):495–498.
Journal cover image

Published In

Amino Acids

DOI

ISSN

0939-4451

Publication Date

June 2006

Volume

30

Issue

4

Start / End Page

495 / 498

Location

Austria

Related Subject Headings

  • Triazines
  • Sensitivity and Specificity
  • Kidney
  • Immunoblotting
  • Humans
  • Hemin
  • Glutathione Transferase
  • Glutathione
  • Ethacrynic Acid
  • Enzyme Inhibitors