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High-speed label-free functional photoacoustic microscopy of mouse brain in action.

Publication ,  Journal Article
Yao, J; Wang, L; Yang, J-M; Maslov, KI; Wong, TTW; Li, L; Huang, C-H; Zou, J; Wang, LV
Published in: Nature methods
May 2015

We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.

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Published In

Nature methods

DOI

EISSN

1548-7105

ISSN

1548-7091

Publication Date

May 2015

Volume

12

Issue

5

Start / End Page

407 / 410

Related Subject Headings

  • Reproducibility of Results
  • Photoacoustic Techniques
  • Microscopy
  • Mice
  • Female
  • Electric Stimulation
  • Developmental Biology
  • Brain
  • Blood Flow Velocity
  • Animals
 

Citation

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Chicago
ICMJE
MLA
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Yao, J., Wang, L., Yang, J.-M., Maslov, K. I., Wong, T. T. W., Li, L., … Wang, L. V. (2015). High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature Methods, 12(5), 407–410. https://doi.org/10.1038/nmeth.3336
Yao, Junjie, Lidai Wang, Joon-Mo Yang, Konstantin I. Maslov, Terence T. W. Wong, Lei Li, Chih-Hsien Huang, Jun Zou, and Lihong V. Wang. “High-speed label-free functional photoacoustic microscopy of mouse brain in action.Nature Methods 12, no. 5 (May 2015): 407–10. https://doi.org/10.1038/nmeth.3336.
Yao J, Wang L, Yang J-M, Maslov KI, Wong TTW, Li L, et al. High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature methods. 2015 May;12(5):407–10.
Yao, Junjie, et al. “High-speed label-free functional photoacoustic microscopy of mouse brain in action.Nature Methods, vol. 12, no. 5, May 2015, pp. 407–10. Epmc, doi:10.1038/nmeth.3336.
Yao J, Wang L, Yang J-M, Maslov KI, Wong TTW, Li L, Huang C-H, Zou J, Wang LV. High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nature methods. 2015 May;12(5):407–410.

Published In

Nature methods

DOI

EISSN

1548-7105

ISSN

1548-7091

Publication Date

May 2015

Volume

12

Issue

5

Start / End Page

407 / 410

Related Subject Headings

  • Reproducibility of Results
  • Photoacoustic Techniques
  • Microscopy
  • Mice
  • Female
  • Electric Stimulation
  • Developmental Biology
  • Brain
  • Blood Flow Velocity
  • Animals