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Influence of pulling handles and device stiffness in single-molecule force spectroscopy.

Publication ,  Journal Article
Maitra, A; Arya, G
Published in: Physical chemistry chemical physics : PCCP
February 2011

In single-molecule force spectroscopy, individual molecules and complexes are often stretched by pulling devices via intervening molecular handles. Accurate interpretation of measurements from such experiments in terms of the underlying energy landscape, defined by activation barriers and intrinsic rates of transition, relies on our understanding, and proper theoretical treatment, of the effects of the pulling device and handle. Here, we present a framework based on Kramers' theory that elucidates the dependence of measured rupture forces and rates on the pulling device stiffness and attributes of the handle, contour length and persistence length. We also introduce a simple analytic model that improves prediction of activation barriers and intrinsic rates for all device stiffnesses and handle properties, thus allowing for a more reliable interpretation of experiments. Our analyses also suggests intuitive ways of displaying the measured force spectra for proper prognosis of device and handle effects and provides the range of device and handle attributes over which these effects can be neglected.

Duke Scholars

Published In

Physical chemistry chemical physics : PCCP

DOI

EISSN

1463-9084

ISSN

1463-9076

Publication Date

February 2011

Volume

13

Issue

5

Start / End Page

1836 / 1842

Related Subject Headings

  • Models, Molecular
  • Microscopy, Atomic Force
  • Mechanical Phenomena
  • Kinetics
  • Chemical Physics
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences
 

Citation

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Maitra, A., & Arya, G. (2011). Influence of pulling handles and device stiffness in single-molecule force spectroscopy. Physical Chemistry Chemical Physics : PCCP, 13(5), 1836–1842. https://doi.org/10.1039/c0cp01528h
Maitra, Arijit, and Gaurav Arya. “Influence of pulling handles and device stiffness in single-molecule force spectroscopy.Physical Chemistry Chemical Physics : PCCP 13, no. 5 (February 2011): 1836–42. https://doi.org/10.1039/c0cp01528h.
Maitra A, Arya G. Influence of pulling handles and device stiffness in single-molecule force spectroscopy. Physical chemistry chemical physics : PCCP. 2011 Feb;13(5):1836–42.
Maitra, Arijit, and Gaurav Arya. “Influence of pulling handles and device stiffness in single-molecule force spectroscopy.Physical Chemistry Chemical Physics : PCCP, vol. 13, no. 5, Feb. 2011, pp. 1836–42. Epmc, doi:10.1039/c0cp01528h.
Maitra A, Arya G. Influence of pulling handles and device stiffness in single-molecule force spectroscopy. Physical chemistry chemical physics : PCCP. 2011 Feb;13(5):1836–1842.
Journal cover image

Published In

Physical chemistry chemical physics : PCCP

DOI

EISSN

1463-9084

ISSN

1463-9076

Publication Date

February 2011

Volume

13

Issue

5

Start / End Page

1836 / 1842

Related Subject Headings

  • Models, Molecular
  • Microscopy, Atomic Force
  • Mechanical Phenomena
  • Kinetics
  • Chemical Physics
  • 51 Physical sciences
  • 40 Engineering
  • 34 Chemical sciences
  • 09 Engineering
  • 03 Chemical Sciences