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Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors.

Publication ,  Journal Article
Xu, C; Liu, X; Geng, Y; Bai, Q; Pan, C; Sun, Y; Chen, X; Yu, H; Wu, Y; Zhang, P; Wu, W; Wang, Y; Wu, Z; Zhang, J; Wang, Z; Yang, R; He, Y ...
Published in: Oncotarget
July 28, 2017

Diffuse intrinsic pontine glioma (DIPG) is a devastating brain tumor, with a median survival of less than one year. Due to enormous difficulties in the acquisition of DIPG specimens and the sophisticated technique required to perform brainstem orthotopic injection, only a handful of DIPG pre-clinical models are available. In this study, we successfully established eight patient-derived DIPG cell lines, mostly derived from treatment-naïve surgery or biopsy specimens. These patient-derived cell lines can be stably passaged in serum-free neural stem cell media and displayed distinct morphologies, growth rates and chromosome abnormalities. In addition, these cells retained genomic hallmarks identical to original human DIPG tumors. Notably, expression of several neural stem cell lineage markers was observed in DIPG cell lines. Moreover, three out of eight cell lines can form orthotopic tumors in mouse brainstem by stereotactic injection and these tumors faithfully represented the characteristics of human DIPG by magnetic resonance imaging (MRI) and histopathological staining. Taken together, we established DIPG pre-clinical models resembling human DIPG and they provided a valuable resource for future biological and therapeutic studies.

Duke Scholars

Published In

Oncotarget

DOI

EISSN

1949-2553

Publication Date

July 28, 2017

Location

United States

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis
 

Citation

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Xu, C., Liu, X., Geng, Y., Bai, Q., Pan, C., Sun, Y., … Zhang, L. (2017). Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors. Oncotarget. https://doi.org/10.18632/oncotarget.19656
Xu, Cheng, Xiaoqing Liu, Yibo Geng, Qingran Bai, Changcun Pan, Yu Sun, Xin Chen, et al. “Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors.Oncotarget, July 28, 2017. https://doi.org/10.18632/oncotarget.19656.
Xu C, Liu X, Geng Y, Bai Q, Pan C, Sun Y, et al. Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors. Oncotarget. 2017 Jul 28;
Xu, Cheng, et al. “Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors.Oncotarget, July 2017. Pubmed, doi:10.18632/oncotarget.19656.
Xu C, Liu X, Geng Y, Bai Q, Pan C, Sun Y, Chen X, Yu H, Wu Y, Zhang P, Wu W, Wang Y, Wu Z, Zhang J, Wang Z, Yang R, Lewis J, Bigner D, Zhao F, He Y, Yan H, Shen Q, Zhang L. Patient-derived DIPG cells preserve stem-like characteristics and generate orthotopic tumors. Oncotarget. 2017 Jul 28;

Published In

Oncotarget

DOI

EISSN

1949-2553

Publication Date

July 28, 2017

Location

United States

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 1112 Oncology and Carcinogenesis