Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges
Publication
, Conference
Castro-Ceseña, AB; Novitskaya, EE; Phadke, A; Varghese, S; McKittrick, J
Published in: Conference Proceedings of the Society for Experimental Mechanics Series
January 1, 2013
Fabrication of collagen sponges from type I collagen isolated from cortical bovine femur bone is reported. Collagen was extracted with 0.1 M EDTA by refreshing the solution every 24 h for 9 days. Sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) demonstrated the isolation of intact pure type I collagen. The sponges were fabricated by freeze-drying and heat-dehydration cross-linking of collagen solution. The resulting sponges showed fibrils arranged in a highly porous network. These sponges can be used for tissue engineering as well as for bone-like composite fabrication. © The Society for Experimental Mechanics, Inc. 2013.
Duke Scholars
Published In
Conference Proceedings of the Society for Experimental Mechanics Series
DOI
EISSN
2191-5652
ISSN
2191-5644
ISBN
9781461444268
Publication Date
January 1, 2013
Volume
5
Start / End Page
73 / 78
Citation
APA
Chicago
ICMJE
MLA
NLM
Castro-Ceseña, A. B., Novitskaya, E. E., Phadke, A., Varghese, S., & McKittrick, J. (2013). Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges. In Conference Proceedings of the Society for Experimental Mechanics Series (Vol. 5, pp. 73–78). https://doi.org/10.1007/978-1-4614-4427-5_11
Castro-Ceseña, A. B., E. E. Novitskaya, A. Phadke, S. Varghese, and J. McKittrick. “Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges.” In Conference Proceedings of the Society for Experimental Mechanics Series, 5:73–78, 2013. https://doi.org/10.1007/978-1-4614-4427-5_11.
Castro-Ceseña AB, Novitskaya EE, Phadke A, Varghese S, McKittrick J. Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges. In: Conference Proceedings of the Society for Experimental Mechanics Series. 2013. p. 73–8.
Castro-Ceseña, A. B., et al. “Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges.” Conference Proceedings of the Society for Experimental Mechanics Series, vol. 5, 2013, pp. 73–78. Scopus, doi:10.1007/978-1-4614-4427-5_11.
Castro-Ceseña AB, Novitskaya EE, Phadke A, Varghese S, McKittrick J. Isolation of collagen from cortical bovine bone for preparation of porous collagen sponges. Conference Proceedings of the Society for Experimental Mechanics Series. 2013. p. 73–78.
Published In
Conference Proceedings of the Society for Experimental Mechanics Series
DOI
EISSN
2191-5652
ISSN
2191-5644
ISBN
9781461444268
Publication Date
January 1, 2013
Volume
5
Start / End Page
73 / 78