Skip to main content
Journal cover image

Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells.

Publication ,  Journal Article
Varghese, S; Hwang, NS; Canver, AC; Theprungsirikul, P; Lin, DW; Elisseeff, J
Published in: Matrix Biol
January 2008

Bone marrow-derived mesenchymal stem cells (MSCs) have strong potential in regeneration of musculoskeletal tissues including cartilage and bone. The microenvironment, comprising of scaffold and soluble factors, plays a pivotal role in determining the efficacy of cartilage tissue regeneration from MSCs. In this study, we investigated the effect of a three-dimensional synthetic-biological composite hydrogel scaffold comprised of poly (ethylene glycol) (PEG) and chondroitin sulfate (CS) on chondrogenesis of MSCs. The cells in CS-based bioactive hydrogels aggregated in a fashion which mimicked the mesenchymal condensation and produced cartilaginous tissues with characteristic morphology and basophilic extracellular matrix production. The aggregation of cells resulted in an enhancement of both chondrogenic gene expressions and cartilage specific matrix production compared to control PEG hydrogels containing no CS-moieties. Moreover, a significant down-regulation of type X collagen expression was observed in PEG/CS hydrogels, indicating that CS inhibits the further differentiation of MSCs into hypertrophic chondrocytes. Overall, this study demonstrates the morphogenetic role of bioactive scaffold-mediated microenvironment on temporal pattern of cartilage specific gene expressions and subsequent matrix production during MSC chondrogenesis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Matrix Biol

DOI

ISSN

0945-053X

Publication Date

January 2008

Volume

27

Issue

1

Start / End Page

12 / 21

Location

Netherlands

Related Subject Headings

  • Tissue Scaffolds
  • Polyethylene Glycols
  • Molecular Structure
  • Mesenchymal Stem Cells
  • Hydrogels
  • Goats
  • Gene Expression Profiling
  • Extracellular Matrix
  • Chondroitin Sulfates
  • Chondrogenesis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Varghese, S., Hwang, N. S., Canver, A. C., Theprungsirikul, P., Lin, D. W., & Elisseeff, J. (2008). Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells. Matrix Biol, 27(1), 12–21. https://doi.org/10.1016/j.matbio.2007.07.002
Varghese, Shyni, Nathaniel S. Hwang, Adam C. Canver, Parnduangji Theprungsirikul, Debora W. Lin, and Jennifer Elisseeff. “Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells.Matrix Biol 27, no. 1 (January 2008): 12–21. https://doi.org/10.1016/j.matbio.2007.07.002.
Varghese S, Hwang NS, Canver AC, Theprungsirikul P, Lin DW, Elisseeff J. Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells. Matrix Biol. 2008 Jan;27(1):12–21.
Varghese, Shyni, et al. “Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells.Matrix Biol, vol. 27, no. 1, Jan. 2008, pp. 12–21. Pubmed, doi:10.1016/j.matbio.2007.07.002.
Varghese S, Hwang NS, Canver AC, Theprungsirikul P, Lin DW, Elisseeff J. Chondroitin sulfate based niches for chondrogenic differentiation of mesenchymal stem cells. Matrix Biol. 2008 Jan;27(1):12–21.
Journal cover image

Published In

Matrix Biol

DOI

ISSN

0945-053X

Publication Date

January 2008

Volume

27

Issue

1

Start / End Page

12 / 21

Location

Netherlands

Related Subject Headings

  • Tissue Scaffolds
  • Polyethylene Glycols
  • Molecular Structure
  • Mesenchymal Stem Cells
  • Hydrogels
  • Goats
  • Gene Expression Profiling
  • Extracellular Matrix
  • Chondroitin Sulfates
  • Chondrogenesis