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Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells.

Publication ,  Journal Article
Hwang, NS; Varghese, S; Puleo, C; Zhang, Z; Elisseeff, J
Published in: J Cell Physiol
August 2007

Mesenchymal stem cells (MSCs) are potentially useful cells for musculoskeletal tissue engineering. However, controlling MSC differentiation and tissue formation in vivo remains a challenge. There is a significant need for well-defined and efficient protocols for directing MSC behaviors in vivo. We hypothesize that morphogenetic signals from chondrocytes may regulate MSC differentiation. In micromass culture of MSCs, incubation with chondrocyte-conditioned medium (CCM) significantly enhanced the production of cartilage specific matrix including type II collagen. In addition, incubation of MSCs with conditioned medium supplemented with osteogenic factors induced more osteogenesis and accumulation of calcium and increased ALP activity. These findings reveal that chondrocyte-secreted factors promote chondrogenesis as well as osteogenesis of MSCs during in vitro micromass culture. Moreover, when MSCs expanded with chondrocyte-conditioned medium were encapsulated in hydrogels and subsequently implanted into athymic mice, basophilic extracellular matrix deposition characteristic of neocartilage was evident. These results indicate that articular chondrocytes produce suitable morphogenetic factors that induce the differentiation program of MSCs in vitro and in vivo.

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Published In

J Cell Physiol

DOI

ISSN

0021-9541

Publication Date

August 2007

Volume

212

Issue

2

Start / End Page

281 / 284

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Signal Transduction
  • Proteoglycans
  • Paracrine Communication
  • Osteogenesis
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Hydrogels
 

Citation

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Hwang, N. S., Varghese, S., Puleo, C., Zhang, Z., & Elisseeff, J. (2007). Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. J Cell Physiol, 212(2), 281–284. https://doi.org/10.1002/jcp.21052
Hwang, Nathaniel S., Shyni Varghese, Christopher Puleo, Zijun Zhang, and Jennifer Elisseeff. “Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells.J Cell Physiol 212, no. 2 (August 2007): 281–84. https://doi.org/10.1002/jcp.21052.
Hwang NS, Varghese S, Puleo C, Zhang Z, Elisseeff J. Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. J Cell Physiol. 2007 Aug;212(2):281–4.
Hwang, Nathaniel S., et al. “Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells.J Cell Physiol, vol. 212, no. 2, Aug. 2007, pp. 281–84. Pubmed, doi:10.1002/jcp.21052.
Hwang NS, Varghese S, Puleo C, Zhang Z, Elisseeff J. Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. J Cell Physiol. 2007 Aug;212(2):281–284.
Journal cover image

Published In

J Cell Physiol

DOI

ISSN

0021-9541

Publication Date

August 2007

Volume

212

Issue

2

Start / End Page

281 / 284

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Signal Transduction
  • Proteoglycans
  • Paracrine Communication
  • Osteogenesis
  • Mice, Nude
  • Mice
  • Mesenchymal Stem Cells
  • Mesenchymal Stem Cell Transplantation
  • Hydrogels