Skip to main content
Journal cover image

Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine.

Publication ,  Journal Article
Hwang, NS; Varghese, S; Theprungsirikul, P; Canver, A; Elisseeff, J
Published in: Biomaterials
December 2006

Differentiation of embryonic stem (ES) cells generally occurs after formation of three-dimensional cell aggregates, known as embryoid bodies (EBs). We have previously reported that hydrogels provide EBs a supportive environment for in vitro chondrogenic differentiation and three dimensional tissue formation [Hwang NS, et al. The Effects of three dimensional culture and growth factors on the chondrogenic differentiation of murine ES cells. Stem Cells 2006;24:284-91]. In this study, we report chondrogenic differentiation of murine ES cells encapsulated in photopolymerizing poly(ethylene-glycol)-based (PEG) hydrogels in the presence of glucosamine (GlcN), an amino monosaccharide found in chitin, glycoproteins and glycosaminoglycans such as hyaluronic acid, chondroitin sulfate and heparin sulfate. We examined the growth and differentiation of encapsulated EBs in standard chondrogenic differentiation medium containing 0-, 2-, and 10-mm GlcN. Morphometric analysis and examination of gene and protein expression indicated that treatment of hydrogel cultures with 2-mm GlcN for 21 days significantly increased EB size, levels of aggrecan mRNA, and tissue-specific extracellular matrix accumulation. GlcN can induce multiple aspects of cell behavior and optimal GlcN concentrations can be beneficial for directing the differentiation and tissue formation of ES cells.

Duke Scholars

Published In

Biomaterials

DOI

ISSN

0142-9612

Publication Date

December 2006

Volume

27

Issue

36

Start / End Page

6015 / 6023

Location

Netherlands

Related Subject Headings

  • Tissue Engineering
  • Stem Cells
  • Mice
  • Hydrogels
  • Glucosamine
  • Dose-Response Relationship, Drug
  • Chondrogenesis
  • Chondrocytes
  • Cell Proliferation
  • Cell Line
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hwang, N. S., Varghese, S., Theprungsirikul, P., Canver, A., & Elisseeff, J. (2006). Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine. Biomaterials, 27(36), 6015–6023. https://doi.org/10.1016/j.biomaterials.2006.06.033
Hwang, Nathaniel S., Shyni Varghese, Parnduangjai Theprungsirikul, Adam Canver, and Jennifer Elisseeff. “Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine.Biomaterials 27, no. 36 (December 2006): 6015–23. https://doi.org/10.1016/j.biomaterials.2006.06.033.
Hwang NS, Varghese S, Theprungsirikul P, Canver A, Elisseeff J. Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine. Biomaterials. 2006 Dec;27(36):6015–23.
Hwang, Nathaniel S., et al. “Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine.Biomaterials, vol. 27, no. 36, Dec. 2006, pp. 6015–23. Pubmed, doi:10.1016/j.biomaterials.2006.06.033.
Hwang NS, Varghese S, Theprungsirikul P, Canver A, Elisseeff J. Enhanced chondrogenic differentiation of murine embryonic stem cells in hydrogels with glucosamine. Biomaterials. 2006 Dec;27(36):6015–6023.
Journal cover image

Published In

Biomaterials

DOI

ISSN

0142-9612

Publication Date

December 2006

Volume

27

Issue

36

Start / End Page

6015 / 6023

Location

Netherlands

Related Subject Headings

  • Tissue Engineering
  • Stem Cells
  • Mice
  • Hydrogels
  • Glucosamine
  • Dose-Response Relationship, Drug
  • Chondrogenesis
  • Chondrocytes
  • Cell Proliferation
  • Cell Line