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Influence of physical properties of biomaterials on cellular behavior.

Publication ,  Journal Article
Lin, S; Sangaj, N; Razafiarison, T; Zhang, C; Varghese, S
Published in: Pharm Res
June 2011

PURPOSE: In this study, we evaluated the effect of hydrogel structural properties on proliferation and biosynthesis activity of encapsulated chondrocytes. METHODS: Hydrogels with varying structural and mechanical properties were prepared by photopolymerizing PEGDA precursors having MWs of 3.4 kDa, 6 kDa, 10 kDa, and 20 kDa and were characterized for their swelling ratio, network structure, morphology, and mechanical properties. The effect of hydrogel structural properties on the cellular activity of encapsulated chondrocytes was studied over four weeks. RESULTS: Varying the molecular weight of PEGDA precursors exhibited a significant effect on the structural and mechanical properties of the hydrogels. Large mesh size was found to support cell proliferation. However, extracellular matrix (ECM) accumulation varied with the precursor molecular weight. Both PEGDA 6 kDa and 10 kDa hydrogels supported GAG accumulation, while PEGDA 10 kDa and 20 KDa hydrogels supported collagen accumulation. Chondrocytes cultured in PEGDA 10 kDa hydrogels expressed a relative increase in collagen type II and aggrecan expression while maintaining low collagen type I expression. CONCLUSIONS: Increasing mesh size of the hydrogels resulted in an increase in cellular proliferation exhibiting the strong correlation between mesh size and cell growth, while mesh size had a differential effect on ECM accumulation and expression of cartilage specific markers.

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Published In

Pharm Res

DOI

EISSN

1573-904X

Publication Date

June 2011

Volume

28

Issue

6

Start / End Page

1422 / 1430

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Tissue Culture Techniques
  • Structure-Activity Relationship
  • Polyethylene Glycols
  • Pharmacology & Pharmacy
  • Molecular Weight
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Glycosaminoglycans
  • Extracellular Matrix
  • Collagen Type II
 

Citation

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Lin, S., Sangaj, N., Razafiarison, T., Zhang, C., & Varghese, S. (2011). Influence of physical properties of biomaterials on cellular behavior. Pharm Res, 28(6), 1422–1430. https://doi.org/10.1007/s11095-011-0378-9
Lin, Susan, Nivedita Sangaj, Tojo Razafiarison, Chao Zhang, and Shyni Varghese. “Influence of physical properties of biomaterials on cellular behavior.Pharm Res 28, no. 6 (June 2011): 1422–30. https://doi.org/10.1007/s11095-011-0378-9.
Lin S, Sangaj N, Razafiarison T, Zhang C, Varghese S. Influence of physical properties of biomaterials on cellular behavior. Pharm Res. 2011 Jun;28(6):1422–30.
Lin, Susan, et al. “Influence of physical properties of biomaterials on cellular behavior.Pharm Res, vol. 28, no. 6, June 2011, pp. 1422–30. Pubmed, doi:10.1007/s11095-011-0378-9.
Lin S, Sangaj N, Razafiarison T, Zhang C, Varghese S. Influence of physical properties of biomaterials on cellular behavior. Pharm Res. 2011 Jun;28(6):1422–1430.
Journal cover image

Published In

Pharm Res

DOI

EISSN

1573-904X

Publication Date

June 2011

Volume

28

Issue

6

Start / End Page

1422 / 1430

Location

United States

Related Subject Headings

  • Tissue Engineering
  • Tissue Culture Techniques
  • Structure-Activity Relationship
  • Polyethylene Glycols
  • Pharmacology & Pharmacy
  • Molecular Weight
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Glycosaminoglycans
  • Extracellular Matrix
  • Collagen Type II