Impact of human CA8 on thermal antinociception in relation to morphine equivalence in mice.
Recently, we showed that murine dorsal root ganglion (DRG) Car8 expression is a cis-regulated eQTL that determines analgesic responses. In this report, we show that transduction through sciatic nerve injection of DRG with human wild-type carbonic anhydrase-8 using adeno-associated virus viral particles (AAV8-V5-CA8WT) produces analgesia in naive male C57BL/6J mice and antihyperalgesia after carrageenan treatment. A peak mean increase of about 4 s in thermal hindpaw withdrawal latency equaled increases in thermal withdrawal latency produced by 10 mg/kg intraperitoneal morphine in these mice. Allometric conversion of this intraperitoneal morphine dose in mice equals an oral morphine dose of about 146 mg in a 60-kg adult. Our work quantifies for the first time analgesia and antihyperalgesia in an inflammatory pain model after DRG transduction by CA8 gene therapy.
Duke Scholars
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Related Subject Headings
- Transduction, Genetic
- Reaction Time
- Pain Threshold
- Pain Measurement
- Pain Management
- Pain
- Neurology & Neurosurgery
- Morphine
- Mice, Inbred C57BL
- Mice
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transduction, Genetic
- Reaction Time
- Pain Threshold
- Pain Measurement
- Pain Management
- Pain
- Neurology & Neurosurgery
- Morphine
- Mice, Inbred C57BL
- Mice