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Correcting the limited view in optical-resolution photoacoustic microscopy.

Publication ,  Journal Article
Liu, W; Zhou, Y; Wang, M; Li, L; Vienneau, E; Chen, R; Luo, J; Xu, C; Zhou, Q; Wang, LV; Yao, J
Published in: Journal of biophotonics
February 2018

Optical-resolution photoacoustic microscopy (OR-PAM) has proven useful for anatomical and functional imaging with high spatial resolutions. However, the coherent signal generation and the desired reflection-mode detection in OR-PAM can result in a limited detectability of features aligned with the acoustic axis (ie, vertical structures). Here, we investigated the limited-view phenomenon in OR-PAM by simulating the generation and propagation of the acoustic pressure waves and determined the key optical parameters affecting the visibility of vertical structures. Proof-of-concept numerical experiments were performed with different illumination angles, optical foci and numerical apertures (NA) of the objective lens. The results collectively show that an NA of 0.3 can readily improve the visibility of vertical structures in a typical reflection-mode OR-PAM system. This conclusion was confirmed by numerical simulations on the cortical blood vessels in a mouse brain and by experiments in a suture-cross phantom and in a mouse brain in vivo.

Duke Scholars

Published In

Journal of biophotonics

DOI

EISSN

1864-0648

ISSN

1864-063X

Publication Date

February 2018

Volume

11

Issue

2

Related Subject Headings

  • Signal-To-Noise Ratio
  • Photoacoustic Techniques
  • Optoelectronics & Photonics
  • Microscopy
  • Mice
  • Image Processing, Computer-Assisted
  • Brain
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3401 Analytical chemistry
 

Citation

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Liu, W., Zhou, Y., Wang, M., Li, L., Vienneau, E., Chen, R., … Yao, J. (2018). Correcting the limited view in optical-resolution photoacoustic microscopy. Journal of Biophotonics, 11(2). https://doi.org/10.1002/jbio.201700196
Liu, Wei, Yuan Zhou, Mengran Wang, Lei Li, Emelina Vienneau, Ruimin Chen, Jianwen Luo, et al. “Correcting the limited view in optical-resolution photoacoustic microscopy.Journal of Biophotonics 11, no. 2 (February 2018). https://doi.org/10.1002/jbio.201700196.
Liu W, Zhou Y, Wang M, Li L, Vienneau E, Chen R, et al. Correcting the limited view in optical-resolution photoacoustic microscopy. Journal of biophotonics. 2018 Feb;11(2).
Liu, Wei, et al. “Correcting the limited view in optical-resolution photoacoustic microscopy.Journal of Biophotonics, vol. 11, no. 2, Feb. 2018. Epmc, doi:10.1002/jbio.201700196.
Liu W, Zhou Y, Wang M, Li L, Vienneau E, Chen R, Luo J, Xu C, Zhou Q, Wang LV, Yao J. Correcting the limited view in optical-resolution photoacoustic microscopy. Journal of biophotonics. 2018 Feb;11(2).
Journal cover image

Published In

Journal of biophotonics

DOI

EISSN

1864-0648

ISSN

1864-063X

Publication Date

February 2018

Volume

11

Issue

2

Related Subject Headings

  • Signal-To-Noise Ratio
  • Photoacoustic Techniques
  • Optoelectronics & Photonics
  • Microscopy
  • Mice
  • Image Processing, Computer-Assisted
  • Brain
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3401 Analytical chemistry