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Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections.

Publication ,  Journal Article
Yiu, G; Wang, Z; Munevar, C; Tieu, E; Shibata, B; Wong, B; Cunefare, D; Farsiu, S; Roberts, J; Thomasy, SM
Published in: Experimental eye research
March 2018

Nonhuman primates are important preclinical models of retinal diseases because they uniquely possess a macula similar to humans. Ocular imaging technologies such as spectral-domain optical coherence tomography (SD-OCT) allow noninvasive, in vivo measurements of chorioretinal layers with near-histological resolution. However, the boundaries are based on differences in reflectivity, and detailed correlations with histological tissue layers have not been explored in rhesus macaques, which are widely used for biomedical research. Here, we compare the macular anatomy and thickness measurements of chorioretinal layers in rhesus macaque eyes using SD-OCT and high-resolution histological sections. Images were obtained from methylmethacrylate-embedded histological sections of 6 healthy adult rhesus macaques, and compared with SD-OCT images from 6 age-matched animals. Thicknesses of chorioretinal layers were measured across the central 3 mm macular region using custom semi-automated or manual software segmentation, and compared between the two modalities. We found that histological sections provide better distinction between the ganglion cell layer (GCL) and inner plexiform layer (IPL) than SD-OCT imaging. The first hyperreflective band between the external limiting membrane (ELM) and retinal pigment epithelium (RPE) appears wider on SD-OCT than the junction between photoreceptor inner and outer segments seen on histology. SD-OCT poorly distinguishes Henle nerve fibers from the outer nuclear layer (ONL), while histology correctly identifies these fibers as part of the outer plexiform layer (OPL). Overall, the GCL, inner nuclear layer (INL), and OPL are significantly thicker on histology, especially at the fovea; while the ONL, choriocapillaris (CC), and outer choroid (OC) are thicker on SD-OCT. Our results show that both SD-OCT and high-resolution histological sections allow reliable measurements of chorioretinal layers in rhesus macaques, with distinct advantages for different sublayers. These findings demonstrate the effects of tissue processing on chorioretinal anatomy, and provide normative values for chorioretinal thickness measurements on SD-OCT for future studies of disease models in these nonhuman primates.

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Published In

Experimental eye research

DOI

EISSN

1096-0007

ISSN

0014-4835

Publication Date

March 2018

Volume

168

Start / End Page

69 / 76

Related Subject Headings

  • Tomography, Optical Coherence
  • Retinal Ganglion Cells
  • Retina
  • Reproducibility of Results
  • Ophthalmology & Optometry
  • Male
  • Macaca mulatta
  • Histological Techniques
  • Female
  • Choroid
 

Citation

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ICMJE
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Yiu, G., Wang, Z., Munevar, C., Tieu, E., Shibata, B., Wong, B., … Thomasy, S. M. (2018). Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections. Experimental Eye Research, 168, 69–76. https://doi.org/10.1016/j.exer.2018.01.012
Yiu, Glenn, Zhe Wang, Christian Munevar, Eric Tieu, Bradley Shibata, Brittany Wong, David Cunefare, Sina Farsiu, Jeffrey Roberts, and Sara M. Thomasy. “Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections.Experimental Eye Research 168 (March 2018): 69–76. https://doi.org/10.1016/j.exer.2018.01.012.
Yiu G, Wang Z, Munevar C, Tieu E, Shibata B, Wong B, et al. Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections. Experimental eye research. 2018 Mar;168:69–76.
Yiu, Glenn, et al. “Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections.Experimental Eye Research, vol. 168, Mar. 2018, pp. 69–76. Epmc, doi:10.1016/j.exer.2018.01.012.
Yiu G, Wang Z, Munevar C, Tieu E, Shibata B, Wong B, Cunefare D, Farsiu S, Roberts J, Thomasy SM. Comparison of chorioretinal layers in rhesus macaques using spectral-domain optical coherence tomography and high-resolution histological sections. Experimental eye research. 2018 Mar;168:69–76.
Journal cover image

Published In

Experimental eye research

DOI

EISSN

1096-0007

ISSN

0014-4835

Publication Date

March 2018

Volume

168

Start / End Page

69 / 76

Related Subject Headings

  • Tomography, Optical Coherence
  • Retinal Ganglion Cells
  • Retina
  • Reproducibility of Results
  • Ophthalmology & Optometry
  • Male
  • Macaca mulatta
  • Histological Techniques
  • Female
  • Choroid