CRISPR-based methods for high-throughput annotation of regulatory DNA.
Publication
, Journal Article
Klann, TS; Black, JB; Gersbach, CA
Published in: Current opinion in biotechnology
August 2018
Developments in CRISPR/Cas9-based technologies provide a new paradigm in functional screening of the genome. Conventional screening methods have focused on high-throughput perturbations of the protein-coding genome with technologies such as RNAi. However, equivalent methods for perturbing the non-coding genome have not existed until recently. CRISPR-based screening of genomic DNA has enabled the study of both genes and non-coding gene regulatory elements. Here we review recent progress in assigning function to the non-coding genome using CRISPR-based genomic and epigenomic screens, and discuss the prospects of these technologies to transforming our understanding of genome structure and regulation.
Duke Scholars
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Published In
Current opinion in biotechnology
DOI
EISSN
1879-0429
ISSN
0958-1669
Publication Date
August 2018
Volume
52
Start / End Page
32 / 41
Related Subject Headings
- Single-Cell Analysis
- Regulatory Sequences, Nucleic Acid
- Genomics
- Epigenomics
- DNA
- CRISPR-Cas Systems
- Biotechnology
- 3206 Medical biotechnology
- 3106 Industrial biotechnology
- 3001 Agricultural biotechnology
Citation
APA
Chicago
ICMJE
MLA
NLM
Klann, T. S., Black, J. B., & Gersbach, C. A. (2018). CRISPR-based methods for high-throughput annotation of regulatory DNA. Current Opinion in Biotechnology, 52, 32–41. https://doi.org/10.1016/j.copbio.2018.02.004
Klann, Tyler S., Joshua B. Black, and Charles A. Gersbach. “CRISPR-based methods for high-throughput annotation of regulatory DNA.” Current Opinion in Biotechnology 52 (August 2018): 32–41. https://doi.org/10.1016/j.copbio.2018.02.004.
Klann TS, Black JB, Gersbach CA. CRISPR-based methods for high-throughput annotation of regulatory DNA. Current opinion in biotechnology. 2018 Aug;52:32–41.
Klann, Tyler S., et al. “CRISPR-based methods for high-throughput annotation of regulatory DNA.” Current Opinion in Biotechnology, vol. 52, Aug. 2018, pp. 32–41. Epmc, doi:10.1016/j.copbio.2018.02.004.
Klann TS, Black JB, Gersbach CA. CRISPR-based methods for high-throughput annotation of regulatory DNA. Current opinion in biotechnology. 2018 Aug;52:32–41.
Published In
Current opinion in biotechnology
DOI
EISSN
1879-0429
ISSN
0958-1669
Publication Date
August 2018
Volume
52
Start / End Page
32 / 41
Related Subject Headings
- Single-Cell Analysis
- Regulatory Sequences, Nucleic Acid
- Genomics
- Epigenomics
- DNA
- CRISPR-Cas Systems
- Biotechnology
- 3206 Medical biotechnology
- 3106 Industrial biotechnology
- 3001 Agricultural biotechnology