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Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.

Publication ,  Journal Article
Solano, CM; Wen, Y; Han, H; Collier, JH
Published in: Methods in molecular biology (Clifton, N.J.)
January 2018

The design, formulation, and immunological evaluation of self-assembling peptide materials is relatively straightforward. Indeed, one of the advantages of synthetic self-assembling peptides is that one can progress from initial concept to in vivo testing in a matter of days. However, because these materials are supramolecular, working with them is not without some practical challenges, and subtle changes in design, synthesis, handling, and formulation can affect the materials' immunogenicity. This chapter is intended to communicate some of these practical aspects of working with these materials that are not always enumerated in conventional research papers. Epitope considerations, peptide synthesis, purification, storage, nanofiber formation, quality control, immunological evaluation, and the overall phenotypic characteristics of the immune responses to be expected from these materials are discussed.

Duke Scholars

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2018

Volume

1777

Start / End Page

233 / 248

Related Subject Headings

  • Quality Control
  • Peptides
  • Mice
  • Immunoassay
  • Immunization
  • Immunity
  • Epitopes
  • Drug Design
  • Developmental Biology
  • Animals
 

Citation

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ICMJE
MLA
NLM
Solano, C. M., Wen, Y., Han, H., & Collier, J. H. (2018). Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies. Methods in Molecular Biology (Clifton, N.J.), 1777, 233–248. https://doi.org/10.1007/978-1-4939-7811-3_14
Solano, Carolina Mora, Yi Wen, Huifang Han, and Joel H. Collier. “Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.Methods in Molecular Biology (Clifton, N.J.) 1777 (January 2018): 233–48. https://doi.org/10.1007/978-1-4939-7811-3_14.
Solano CM, Wen Y, Han H, Collier JH. Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies. Methods in molecular biology (Clifton, NJ). 2018 Jan;1777:233–48.
Solano, Carolina Mora, et al. “Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.Methods in Molecular Biology (Clifton, N.J.), vol. 1777, Jan. 2018, pp. 233–48. Epmc, doi:10.1007/978-1-4939-7811-3_14.
Solano CM, Wen Y, Han H, Collier JH. Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies. Methods in molecular biology (Clifton, NJ). 2018 Jan;1777:233–248.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2018

Volume

1777

Start / End Page

233 / 248

Related Subject Headings

  • Quality Control
  • Peptides
  • Mice
  • Immunoassay
  • Immunization
  • Immunity
  • Epitopes
  • Drug Design
  • Developmental Biology
  • Animals