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Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.

Publication ,  Journal Article
Kang, Y; Kuybeda, O; de Waal, PW; Mukherjee, S; Van Eps, N; Dutka, P; Zhou, XE; Bartesaghi, A; Erramilli, S; Morizumi, T; Gu, X; Yin, Y ...
Published in: Nature
June 2018

G-protein-coupled receptors comprise the largest family of mammalian transmembrane receptors. They mediate numerous cellular pathways by coupling with downstream signalling transducers, including the hetrotrimeric G proteins Gs (stimulatory) and Gi (inhibitory) and several arrestin proteins. The structural mechanisms that define how G-protein-coupled receptors selectively couple to a specific type of G protein or arrestin remain unknown. Here, using cryo-electron microscopy, we show that the major interactions between activated rhodopsin and Gi are mediated by the C-terminal helix of the Gi α-subunit, which is wedged into the cytoplasmic cavity of the transmembrane helix bundle and directly contacts the amino terminus of helix 8 of rhodopsin. Structural comparisons of inactive, Gi-bound and arrestin-bound forms of rhodopsin with inactive and Gs-bound forms of the β2-adrenergic receptor provide a foundation to understand the unique structural signatures that are associated with the recognition of Gs, Gi and arrestin by activated G-protein-coupled receptors.

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Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

June 2018

Volume

558

Issue

7711

Start / End Page

553 / 558

Related Subject Headings

  • Substrate Specificity
  • Signal Transduction
  • Rhodopsin
  • Receptors, Adrenergic, beta-2
  • Models, Molecular
  • Humans
  • General Science & Technology
  • GTP-Binding Protein alpha Subunits, Gs
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Cryoelectron Microscopy
 

Citation

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Kang, Y., Kuybeda, O., de Waal, P. W., Mukherjee, S., Van Eps, N., Dutka, P., … Xu, H. E. (2018). Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature, 558(7711), 553–558. https://doi.org/10.1038/s41586-018-0215-y
Kang, Yanyong, Oleg Kuybeda, Parker W. de Waal, Somnath Mukherjee, Ned Van Eps, Przemyslaw Dutka, X Edward Zhou, et al. “Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.Nature 558, no. 7711 (June 2018): 553–58. https://doi.org/10.1038/s41586-018-0215-y.
Kang Y, Kuybeda O, de Waal PW, Mukherjee S, Van Eps N, Dutka P, et al. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature. 2018 Jun;558(7711):553–8.
Kang, Yanyong, et al. “Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.Nature, vol. 558, no. 7711, June 2018, pp. 553–58. Epmc, doi:10.1038/s41586-018-0215-y.
Kang Y, Kuybeda O, de Waal PW, Mukherjee S, Van Eps N, Dutka P, Zhou XE, Bartesaghi A, Erramilli S, Morizumi T, Gu X, Yin Y, Liu P, Jiang Y, Meng X, Zhao G, Melcher K, Ernst OP, Kossiakoff AA, Subramaniam S, Xu HE. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein. Nature. 2018 Jun;558(7711):553–558.
Journal cover image

Published In

Nature

DOI

EISSN

1476-4687

ISSN

0028-0836

Publication Date

June 2018

Volume

558

Issue

7711

Start / End Page

553 / 558

Related Subject Headings

  • Substrate Specificity
  • Signal Transduction
  • Rhodopsin
  • Receptors, Adrenergic, beta-2
  • Models, Molecular
  • Humans
  • General Science & Technology
  • GTP-Binding Protein alpha Subunits, Gs
  • GTP-Binding Protein alpha Subunits, Gi-Go
  • Cryoelectron Microscopy