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Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.

Publication ,  Journal Article
Choi, VW; Asokan, A; Haberman, RA; Samulski, RJ
Published in: Curr Protoc Mol Biol
April 2007

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.

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Published In

Curr Protoc Mol Biol

DOI

EISSN

1934-3647

Publication Date

April 2007

Volume

Chapter 16

Start / End Page

Unit / 16.25

Location

United States

Related Subject Headings

  • Transfection
  • Plasmids
  • Humans
  • Genetic Vectors
  • Gene Transfer Techniques
  • Dependovirus
  • Biochemistry & Molecular Biology
  • Animals
 

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Choi, V. W., Asokan, A., Haberman, R. A., & Samulski, R. J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol, Chapter 16, Unit-16.25. https://doi.org/10.1002/0471142727.mb1625s78
Choi, Vivian W., Aravind Asokan, Rebecca A. Haberman, and Richard Jude Samulski. “Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.Curr Protoc Mol Biol Chapter 16 (April 2007): Unit-16.25. https://doi.org/10.1002/0471142727.mb1625s78.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol. 2007 Apr;Chapter 16:Unit-16.25.
Choi, Vivian W., et al. “Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.Curr Protoc Mol Biol, vol. Chapter 16, Apr. 2007, p. Unit-16.25. Pubmed, doi:10.1002/0471142727.mb1625s78.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol. 2007 Apr;Chapter 16:Unit-16.25.

Published In

Curr Protoc Mol Biol

DOI

EISSN

1934-3647

Publication Date

April 2007

Volume

Chapter 16

Start / End Page

Unit / 16.25

Location

United States

Related Subject Headings

  • Transfection
  • Plasmids
  • Humans
  • Genetic Vectors
  • Gene Transfer Techniques
  • Dependovirus
  • Biochemistry & Molecular Biology
  • Animals