Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.
Publication
, Journal Article
Choi, VW; Asokan, A; Haberman, RA; Samulski, RJ
Published in: Curr Protoc Mol Biol
April 2007
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.
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Published In
Curr Protoc Mol Biol
DOI
EISSN
1934-3647
Publication Date
April 2007
Volume
Chapter 16
Start / End Page
Unit / 16.25
Location
United States
Related Subject Headings
- Transfection
- Plasmids
- Humans
- Genetic Vectors
- Gene Transfer Techniques
- Dependovirus
- Biochemistry & Molecular Biology
- Animals
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Choi, V. W., Asokan, A., Haberman, R. A., & Samulski, R. J. (2007). Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol, Chapter 16, Unit-16.25. https://doi.org/10.1002/0471142727.mb1625s78
Choi, Vivian W., Aravind Asokan, Rebecca A. Haberman, and Richard Jude Samulski. “Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.” Curr Protoc Mol Biol Chapter 16 (April 2007): Unit-16.25. https://doi.org/10.1002/0471142727.mb1625s78.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol. 2007 Apr;Chapter 16:Unit-16.25.
Choi, Vivian W., et al. “Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.” Curr Protoc Mol Biol, vol. Chapter 16, Apr. 2007, p. Unit-16.25. Pubmed, doi:10.1002/0471142727.mb1625s78.
Choi VW, Asokan A, Haberman RA, Samulski RJ. Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol. 2007 Apr;Chapter 16:Unit-16.25.
Published In
Curr Protoc Mol Biol
DOI
EISSN
1934-3647
Publication Date
April 2007
Volume
Chapter 16
Start / End Page
Unit / 16.25
Location
United States
Related Subject Headings
- Transfection
- Plasmids
- Humans
- Genetic Vectors
- Gene Transfer Techniques
- Dependovirus
- Biochemistry & Molecular Biology
- Animals