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Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.

Publication ,  Journal Article
Gray, SJ; Choi, VW; Asokan, A; Haberman, RA; McCown, TJ; Samulski, RJ
Published in: Curr Protoc Neurosci
October 2011

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP.

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Published In

Curr Protoc Neurosci

DOI

EISSN

1934-8576

Publication Date

October 2011

Volume

Chapter 4

Start / End Page

Unit / 4.17

Location

United States

Related Subject Headings

  • Genetic Vectors
  • Genetic Therapy
  • Gene Transfer Techniques
  • Dependovirus
  • Brain
  • Animals
 

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Gray, S. J., Choi, V. W., Asokan, A., Haberman, R. A., McCown, T. J., & Samulski, R. J. (2011). Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. Curr Protoc Neurosci, Chapter 4, Unit-4.17. https://doi.org/10.1002/0471142301.ns0417s57
Gray, Steven J., Vivian W. Choi, Aravind Asokan, Rebecca A. Haberman, Thomas J. McCown, and Richard Jude Samulski. “Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.Curr Protoc Neurosci Chapter 4 (October 2011): Unit-4.17. https://doi.org/10.1002/0471142301.ns0417s57.
Gray SJ, Choi VW, Asokan A, Haberman RA, McCown TJ, Samulski RJ. Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. Curr Protoc Neurosci. 2011 Oct;Chapter 4:Unit-4.17.
Gray, Steven J., et al. “Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.Curr Protoc Neurosci, vol. Chapter 4, Oct. 2011, p. Unit-4.17. Pubmed, doi:10.1002/0471142301.ns0417s57.
Gray SJ, Choi VW, Asokan A, Haberman RA, McCown TJ, Samulski RJ. Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration. Curr Protoc Neurosci. 2011 Oct;Chapter 4:Unit-4.17.

Published In

Curr Protoc Neurosci

DOI

EISSN

1934-8576

Publication Date

October 2011

Volume

Chapter 4

Start / End Page

Unit / 4.17

Location

United States

Related Subject Headings

  • Genetic Vectors
  • Genetic Therapy
  • Gene Transfer Techniques
  • Dependovirus
  • Brain
  • Animals