Systemic gene transfer to skeletal muscle using reengineered AAV vectors.
Publication
, Journal Article
Phillips, JL; Hegge, J; Wolff, JA; Samulski, RJ; Asokan, A
Published in: Methods Mol Biol
2011
Gene therapy of musculoskeletal disorders warrants efficient gene transfer to a wide range of muscle groups. Reengineered adeno-associated viral (AAV) vectors that selectively transduce muscle tissue following systemic administration are attractive candidates for such applications. Here we provide examples of several lab-derived AAV vectors that display systemic tissue tropism in mice. Methods to evaluate the efficiency of gene transfer to skeletal muscle following intravenous or isolated limb infusion of AAV -vectors in mice are discussed in detail.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
709
Start / End Page
141 / 151
Location
United States
Related Subject Headings
- Tropism
- Muscular Dystrophy, Animal
- Muscular Dystrophies
- Muscle, Skeletal
- Mice
- Genetic Vectors
- Genetic Therapy
- Genetic Engineering
- Gene Transfer Techniques
- Disease Models, Animal
Citation
APA
Chicago
ICMJE
MLA
NLM
Phillips, J. L., Hegge, J., Wolff, J. A., Samulski, R. J., & Asokan, A. (2011). Systemic gene transfer to skeletal muscle using reengineered AAV vectors. Methods Mol Biol, 709, 141–151. https://doi.org/10.1007/978-1-61737-982-6_9
Phillips, Jana L., Julia Hegge, Jon A. Wolff, R Jude Samulski, and Aravind Asokan. “Systemic gene transfer to skeletal muscle using reengineered AAV vectors.” Methods Mol Biol 709 (2011): 141–51. https://doi.org/10.1007/978-1-61737-982-6_9.
Phillips JL, Hegge J, Wolff JA, Samulski RJ, Asokan A. Systemic gene transfer to skeletal muscle using reengineered AAV vectors. Methods Mol Biol. 2011;709:141–51.
Phillips, Jana L., et al. “Systemic gene transfer to skeletal muscle using reengineered AAV vectors.” Methods Mol Biol, vol. 709, 2011, pp. 141–51. Pubmed, doi:10.1007/978-1-61737-982-6_9.
Phillips JL, Hegge J, Wolff JA, Samulski RJ, Asokan A. Systemic gene transfer to skeletal muscle using reengineered AAV vectors. Methods Mol Biol. 2011;709:141–151.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2011
Volume
709
Start / End Page
141 / 151
Location
United States
Related Subject Headings
- Tropism
- Muscular Dystrophy, Animal
- Muscular Dystrophies
- Muscle, Skeletal
- Mice
- Genetic Vectors
- Genetic Therapy
- Genetic Engineering
- Gene Transfer Techniques
- Disease Models, Animal