A simple and robust fluorescent labeling method to quantify trophoblast fusion.
Publication
, Journal Article
Zhang, Y; Yang, H
Published in: Placenta
February 2019
Trophoblast fusion into syncytiotrophoblasts is a specialized yet enigmatic cellular process, which is essential for placental development and function. To facilitate mechanistic understanding of this critical process, here we re-purposed a widely used fluorescent membrane potential dye, Di-8-ANEPPS, to stably label the plasma membrane of live BeWo trophoblast cells. Compared to the methods currently available to quantify trophoblast fusion, our new fluorescent labeling method is simple, economical, robust and versatile, enabling quick and accurate quantification of fusion index in living cells.
Duke Scholars
Published In
Placenta
DOI
EISSN
1532-3102
Publication Date
February 2019
Volume
77
Start / End Page
16 / 18
Location
Netherlands
Related Subject Headings
- Trophoblasts
- Pyridinium Compounds
- Pregnancy
- Placentation
- Obstetrics & Reproductive Medicine
- Microscopy, Fluorescence
- Humans
- Fluorescent Dyes
- Female
- Colforsin
Citation
APA
Chicago
ICMJE
MLA
NLM
Zhang, Y., & Yang, H. (2019). A simple and robust fluorescent labeling method to quantify trophoblast fusion. Placenta, 77, 16–18. https://doi.org/10.1016/j.placenta.2019.01.016
Zhang, Yang, and Huanghe Yang. “A simple and robust fluorescent labeling method to quantify trophoblast fusion.” Placenta 77 (February 2019): 16–18. https://doi.org/10.1016/j.placenta.2019.01.016.
Zhang Y, Yang H. A simple and robust fluorescent labeling method to quantify trophoblast fusion. Placenta. 2019 Feb;77:16–8.
Zhang, Yang, and Huanghe Yang. “A simple and robust fluorescent labeling method to quantify trophoblast fusion.” Placenta, vol. 77, Feb. 2019, pp. 16–18. Pubmed, doi:10.1016/j.placenta.2019.01.016.
Zhang Y, Yang H. A simple and robust fluorescent labeling method to quantify trophoblast fusion. Placenta. 2019 Feb;77:16–18.
Published In
Placenta
DOI
EISSN
1532-3102
Publication Date
February 2019
Volume
77
Start / End Page
16 / 18
Location
Netherlands
Related Subject Headings
- Trophoblasts
- Pyridinium Compounds
- Pregnancy
- Placentation
- Obstetrics & Reproductive Medicine
- Microscopy, Fluorescence
- Humans
- Fluorescent Dyes
- Female
- Colforsin