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Neurite orientation dispersion and density imaging of mouse brain microstructure.

Publication ,  Journal Article
Wang, N; Zhang, J; Cofer, G; Qi, Y; Anderson, RJ; White, LE; Allan Johnson, G
Published in: Brain Struct Funct
June 2019

Advanced biophysical models like neurite orientation dispersion and density imaging (NODDI) have been developed to estimate the microstructural complexity of voxels enriched in dendrites and axons for both in vivo and ex vivo studies. NODDI metrics derived from high spatial and angular resolution diffusion MRI using the fixed mouse brain as a reference template have not yet been reported due in part to the extremely long scan time required. In this study, we modified the three-dimensional diffusion-weighted spin-echo pulse sequence for multi-shell and undersampling acquisition to reduce the scan time. This allowed us to acquire several exhaustive datasets that would otherwise not be attainable. NODDI metrics were derived from a complex 8-shell diffusion (1000-8000 s/mm2) dataset with 384 diffusion gradient-encoding directions at 50 µm isotropic resolution. These provided a foundation for exploration of tradeoffs among acquisition parameters. A three-shell acquisition strategy covering low, medium, and high b values with at least angular resolution of 64 is essential for ex vivo NODDI experiments. The good agreement between neurite density index (NDI) and the orientation dispersion index (ODI) with the subsequent histochemical analysis of myelin and neuronal density highlights that NODDI could provide new insight into the microstructure of the brain. Furthermore, we found that NDI is sensitive to microstructural variations in the corpus callosum using a well-established demyelination cuprizone model. The study lays the ground work for developing protocols for routine use of high-resolution NODDI method in characterizing brain microstructure in mouse models.

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Published In

Brain Struct Funct

DOI

EISSN

1863-2661

Publication Date

June 2019

Volume

224

Issue

5

Start / End Page

1797 / 1813

Location

Germany

Related Subject Headings

  • Neurology & Neurosurgery
  • Neurites
  • Mice, Inbred C57BL
  • Male
  • Image Processing, Computer-Assisted
  • Diffusion Tensor Imaging
  • Diffusion Magnetic Resonance Imaging
  • Developmental Biology
  • Dendrites
  • Brain Mapping
 

Citation

APA
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Wang, N., Zhang, J., Cofer, G., Qi, Y., Anderson, R. J., White, L. E., & Allan Johnson, G. (2019). Neurite orientation dispersion and density imaging of mouse brain microstructure. Brain Struct Funct, 224(5), 1797–1813. https://doi.org/10.1007/s00429-019-01877-x
Wang, Nian, Jieying Zhang, Gary Cofer, Yi Qi, Robert J. Anderson, Leonard E. White, and G. Allan Johnson. “Neurite orientation dispersion and density imaging of mouse brain microstructure.Brain Struct Funct 224, no. 5 (June 2019): 1797–1813. https://doi.org/10.1007/s00429-019-01877-x.
Wang N, Zhang J, Cofer G, Qi Y, Anderson RJ, White LE, et al. Neurite orientation dispersion and density imaging of mouse brain microstructure. Brain Struct Funct. 2019 Jun;224(5):1797–813.
Wang, Nian, et al. “Neurite orientation dispersion and density imaging of mouse brain microstructure.Brain Struct Funct, vol. 224, no. 5, June 2019, pp. 1797–813. Pubmed, doi:10.1007/s00429-019-01877-x.
Wang N, Zhang J, Cofer G, Qi Y, Anderson RJ, White LE, Allan Johnson G. Neurite orientation dispersion and density imaging of mouse brain microstructure. Brain Struct Funct. 2019 Jun;224(5):1797–1813.
Journal cover image

Published In

Brain Struct Funct

DOI

EISSN

1863-2661

Publication Date

June 2019

Volume

224

Issue

5

Start / End Page

1797 / 1813

Location

Germany

Related Subject Headings

  • Neurology & Neurosurgery
  • Neurites
  • Mice, Inbred C57BL
  • Male
  • Image Processing, Computer-Assisted
  • Diffusion Tensor Imaging
  • Diffusion Magnetic Resonance Imaging
  • Developmental Biology
  • Dendrites
  • Brain Mapping