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Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity.

Publication ,  Journal Article
Yu, J; Xu, Y; Li, S; Seifert, GV; Becker, ML
Published in: Biomacromolecules
December 2017

Polymer-bioceramic composites incorporate the desirable properties of each material while mitigating the limiting characteristics of each component. 1,6-Hexanediol l-phenylalanine-based poly(ester urea) (PEU) blended with hydroxyapatite (HA) nanocrystals were three-dimensional (3D) printed into porous scaffolds (75% porosity) via fused deposition modeling and seeded with MC3T3-E1 preosteoblast cells in vitro to examine their bioactivity. The resulting 3D printed scaffolds exhibited a compressive modulus of ∼50 MPa after a 1-week incubation in PBS at 37 °C, cell viability >95%, and a composition-dependent enhancement of radio-contrast. The influence of HA on MC3T3-E1 proliferation and differentiation was measured using quantitative real-time polymerase chain reaction, immunohistochemistry and biochemical assays. After 4 weeks, alkaline phosphatase activity increased significantly for the 30% HA composite with values reaching 2.5-fold greater than the control. Bone sialoprotein showed approximately 880-fold higher expression and 15-fold higher expression of osteocalcin on the 30% HA composite compared to those of the control. Calcium quantification results demonstrated a 185-fold increase of calcium concentration in mineralized extracellular matrix deposition after 4 weeks of cell culture in samples with higher HA content. 3D printed HA-containing PEU composites promote bone regeneration and have the potential to be used in orthopedic applications.

Duke Scholars

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Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

December 2017

Volume

18

Issue

12

Start / End Page

4171 / 4183

Related Subject Headings

  • Urea
  • Tissue Scaffolds
  • Tissue Engineering
  • Printing, Three-Dimensional
  • Porosity
  • Polymers
  • Polymers
  • Polyesters
  • Osteogenesis
  • Osteocalcin
 

Citation

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Chicago
ICMJE
MLA
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Yu, J., Xu, Y., Li, S., Seifert, G. V., & Becker, M. L. (2017). Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity. Biomacromolecules, 18(12), 4171–4183. https://doi.org/10.1021/acs.biomac.7b01222
Yu, Jiayi, Yanyi Xu, Shan Li, Gabrielle V. Seifert, and Matthew L. Becker. “Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity.Biomacromolecules 18, no. 12 (December 2017): 4171–83. https://doi.org/10.1021/acs.biomac.7b01222.
Yu J, Xu Y, Li S, Seifert GV, Becker ML. Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity. Biomacromolecules. 2017 Dec;18(12):4171–83.
Yu, Jiayi, et al. “Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity.Biomacromolecules, vol. 18, no. 12, Dec. 2017, pp. 4171–83. Epmc, doi:10.1021/acs.biomac.7b01222.
Yu J, Xu Y, Li S, Seifert GV, Becker ML. Three-Dimensional Printing of Nano Hydroxyapatite/Poly(ester urea) Composite Scaffolds with Enhanced Bioactivity. Biomacromolecules. 2017 Dec;18(12):4171–4183.
Journal cover image

Published In

Biomacromolecules

DOI

EISSN

1526-4602

ISSN

1525-7797

Publication Date

December 2017

Volume

18

Issue

12

Start / End Page

4171 / 4183

Related Subject Headings

  • Urea
  • Tissue Scaffolds
  • Tissue Engineering
  • Printing, Three-Dimensional
  • Porosity
  • Polymers
  • Polymers
  • Polyesters
  • Osteogenesis
  • Osteocalcin