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Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity.

Publication ,  Journal Article
Tang, W; Policastro, GM; Hua, G; Guo, K; Zhou, J; Wesdemiotis, C; Doll, GL; Becker, ML
Published in: Journal of the American Chemical Society
November 2014

A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO2, ZrO2, CeO2, Fe3O4 and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO2-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO2. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

November 2014

Volume

136

Issue

46

Start / End Page

16357 / 16367

Related Subject Headings

  • Titanium
  • Surface Properties
  • Osteogenesis
  • Minerals
  • Mice
  • Intercellular Signaling Peptides and Proteins
  • Immobilized Proteins
  • Hydrogen-Ion Concentration
  • Histones
  • General Chemistry
 

Citation

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Tang, W., Policastro, G. M., Hua, G., Guo, K., Zhou, J., Wesdemiotis, C., … Becker, M. L. (2014). Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity. Journal of the American Chemical Society, 136(46), 16357–16367. https://doi.org/10.1021/ja508946h
Tang, Wen, Gina M. Policastro, Geng Hua, Kai Guo, Jinjun Zhou, Chrys Wesdemiotis, Gary L. Doll, and Matthew L. Becker. “Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity.Journal of the American Chemical Society 136, no. 46 (November 2014): 16357–67. https://doi.org/10.1021/ja508946h.
Tang W, Policastro GM, Hua G, Guo K, Zhou J, Wesdemiotis C, et al. Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity. Journal of the American Chemical Society. 2014 Nov;136(46):16357–67.
Tang, Wen, et al. “Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity.Journal of the American Chemical Society, vol. 136, no. 46, Nov. 2014, pp. 16357–67. Epmc, doi:10.1021/ja508946h.
Tang W, Policastro GM, Hua G, Guo K, Zhou J, Wesdemiotis C, Doll GL, Becker ML. Bioactive surface modification of metal oxides via catechol-bearing modular peptides: multivalent-binding, surface retention, and peptide bioactivity. Journal of the American Chemical Society. 2014 Nov;136(46):16357–16367.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

November 2014

Volume

136

Issue

46

Start / End Page

16357 / 16367

Related Subject Headings

  • Titanium
  • Surface Properties
  • Osteogenesis
  • Minerals
  • Mice
  • Intercellular Signaling Peptides and Proteins
  • Immobilized Proteins
  • Hydrogen-Ion Concentration
  • Histones
  • General Chemistry