Universal gradient substrates for "click" biofunctionalization

A novel and versatile method for fabricating continuously variable concentration gradients of surface-conjugated biomolecules is reported. This technology utilizes graded UV oxidation of self-assembled monolayers (SAM) as well as converts the resulting carboxylate gradient into an increasing density of alkyne functionalities appropriate for click-chemistry surface conjugation of biomolecules. This approach to the fabrication of bioactive surface gradients has been to develop and characterize a functional gradient substrate to which a variety of species can be attached. In the final fabrication step of the Universal Gradient Substrate, a bifunctional propargyl-derivatized linker was attached to the acid gradient by using standard amidation methods to yield a surface possessing an increasing concentration of alkyne groups. cell function. This technology for fabricating continuously variable density gradients of bioactive molecules has a wide range of potential uses in biomaterials.

Duke Scholars

Author Matthew L Becker Chemistry