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Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.

Publication ,  Chapter
Deshpande, S; Yang, Y; Chilkoti, A; Zauscher, S
January 2019

The recognition that nucleic acids can be used as polymeric materials led to the blossoming of the field of DNA nanotechnology, with a broad range of applications in biotechnology, biosensors, diagnostics, and drug delivery. These applications require efficient methods to synthesize and chemically modify high molecular weight DNA. Here, we discuss terminal deoxynucleotidyl transferase (TdT)-catalyzed enzymatic polymerization (TcEP) as an alternative to conventional enzymatic and solid-phase DNA synthesis. We describe biochemical requirements for TcEP and provide step-by-step protocols to carry out TcEP in solution and from surfaces.

Duke Scholars

DOI

Publication Date

January 2019

Volume

627

Start / End Page

163 / 188

Related Subject Headings

  • Polymerization
  • Molecular Weight
  • DNA Nucleotidylexotransferase
  • DNA
  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Deshpande, S., Yang, Y., Chilkoti, A., & Zauscher, S. (2019). Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase. (Vol. 627, pp. 163–188). https://doi.org/10.1016/bs.mie.2019.07.044
Deshpande, Sonal, Yunqi Yang, Ashutosh Chilkoti, and Stefan Zauscher. “Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.,” 627:163–88, 2019. https://doi.org/10.1016/bs.mie.2019.07.044.
Deshpande, Sonal, et al. Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase. Vol. 627, 2019, pp. 163–88. Epmc, doi:10.1016/bs.mie.2019.07.044.

DOI

Publication Date

January 2019

Volume

627

Start / End Page

163 / 188

Related Subject Headings

  • Polymerization
  • Molecular Weight
  • DNA Nucleotidylexotransferase
  • DNA
  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology