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Intrinsically disordered proteins access a range of hysteretic phase separation behaviors.

Publication ,  Journal Article
Garcia Quiroz, F; Li, NK; Roberts, S; Weber, P; Dzuricky, M; Weitzhandler, I; Yingling, YG; Chilkoti, A
Published in: Science advances
October 2019

The phase separation behavior of intrinsically disordered proteins (IDPs) is thought of as analogous to that of polymers that undergo equilibrium lower or upper critical solution temperature (LCST and UCST, respectively) phase transition. This view, however, ignores possible nonequilibrium properties of protein assemblies. Here, by studying IDP polymers (IDPPs) composed of repeat motifs that encode LCST or UCST phase behavior, we discovered that IDPs can access a wide spectrum of nonequilibrium, hysteretic phase behaviors. Experimentally and through simulations, we show that hysteresis in IDPPs is tunable and that it emerges through increasingly stable interchain interactions in the insoluble phase. To explore the utility of hysteretic IDPPs, we engineer self-assembling nanostructures with tunable stability. These findings shine light on the rich phase separation behavior of IDPs and illustrate hysteresis as a design parameter to program nonequilibrium phase behavior in self-assembling materials.

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Published In

Science advances

DOI

EISSN

2375-2548

ISSN

2375-2548

Publication Date

October 2019

Volume

5

Issue

10

Start / End Page

eaax5177

Related Subject Headings

  • Urea
  • Temperature
  • Proline
  • Phase Transition
  • Nanoparticles
  • Molecular Dynamics Simulation
  • Intrinsically Disordered Proteins
  • Hydrophobic and Hydrophilic Interactions
  • Hydrogen Bonding
  • Circular Dichroism
 

Citation

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Garcia Quiroz, F., Li, N. K., Roberts, S., Weber, P., Dzuricky, M., Weitzhandler, I., … Chilkoti, A. (2019). Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. Science Advances, 5(10), eaax5177. https://doi.org/10.1126/sciadv.aax5177
Garcia Quiroz, Felipe, Nan K. Li, Stefan Roberts, Patrick Weber, Michael Dzuricky, Isaac Weitzhandler, Yaroslava G. Yingling, and Ashutosh Chilkoti. “Intrinsically disordered proteins access a range of hysteretic phase separation behaviors.Science Advances 5, no. 10 (October 2019): eaax5177. https://doi.org/10.1126/sciadv.aax5177.
Garcia Quiroz F, Li NK, Roberts S, Weber P, Dzuricky M, Weitzhandler I, et al. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. Science advances. 2019 Oct;5(10):eaax5177.
Garcia Quiroz, Felipe, et al. “Intrinsically disordered proteins access a range of hysteretic phase separation behaviors.Science Advances, vol. 5, no. 10, Oct. 2019, p. eaax5177. Epmc, doi:10.1126/sciadv.aax5177.
Garcia Quiroz F, Li NK, Roberts S, Weber P, Dzuricky M, Weitzhandler I, Yingling YG, Chilkoti A. Intrinsically disordered proteins access a range of hysteretic phase separation behaviors. Science advances. 2019 Oct;5(10):eaax5177.

Published In

Science advances

DOI

EISSN

2375-2548

ISSN

2375-2548

Publication Date

October 2019

Volume

5

Issue

10

Start / End Page

eaax5177

Related Subject Headings

  • Urea
  • Temperature
  • Proline
  • Phase Transition
  • Nanoparticles
  • Molecular Dynamics Simulation
  • Intrinsically Disordered Proteins
  • Hydrophobic and Hydrophilic Interactions
  • Hydrogen Bonding
  • Circular Dichroism