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Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging.

Publication ,  Journal Article
Wang, D; Xu, S; Pant, P; Redington, E; Soltanian-Zadeh, S; Farsiu, S; Gong, Y
Published in: Biomedical optics express
December 2019

Large scale simultaneous recording of fast patterns of neural activity remains challenging. Volumetric imaging modalities such as scanning-beam light-sheet microscopy (LSM) and wide-field light-field microscopy (WFLFM) fall short of the goal due to their complex calibration procedure, low spatial resolution, or high-photobleaching. Here, we demonstrate a hybrid light-sheet light-field microscopy (LSLFM) modality that yields high spatial resolution with simplified alignment of the imaging plane and the excitation plane. This new modality combines the selective excitation of light-sheet illumination with volumetric light-field imaging. This modality overcomes the current limitations of the scanning-beam LSM and WFLFM implementations. Compared with LSM, LSLFM captures volumetric data at a frame rate 50× lower than the rate of LSM and requires no dynamic calibration. Compared with WFLFM, LSLFM produces moderate improvements in spatial resolutions, 10 times improvement in the contrast when imaging fluorescent beads, and 3.2× the signal-to-noise ratio in the detection of neural activity when imaging live zebrafish expressing a genetically encoded calcium sensor.

Duke Scholars

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Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

December 2019

Volume

10

Issue

12

Start / End Page

6595 / 6610

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics
 

Citation

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Wang, D., Xu, S., Pant, P., Redington, E., Soltanian-Zadeh, S., Farsiu, S., & Gong, Y. (2019). Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging. Biomedical Optics Express, 10(12), 6595–6610. https://doi.org/10.1364/boe.10.006595
Wang, Depeng, Stephen Xu, Praruj Pant, Emily Redington, Somayyeh Soltanian-Zadeh, Sina Farsiu, and Yiyang Gong. “Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging.Biomedical Optics Express 10, no. 12 (December 2019): 6595–6610. https://doi.org/10.1364/boe.10.006595.
Wang D, Xu S, Pant P, Redington E, Soltanian-Zadeh S, Farsiu S, et al. Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging. Biomedical optics express. 2019 Dec;10(12):6595–610.
Wang, Depeng, et al. “Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging.Biomedical Optics Express, vol. 10, no. 12, Dec. 2019, pp. 6595–610. Epmc, doi:10.1364/boe.10.006595.
Wang D, Xu S, Pant P, Redington E, Soltanian-Zadeh S, Farsiu S, Gong Y. Hybrid light-sheet and light-field microscope for high resolution and large volume neuroimaging. Biomedical optics express. 2019 Dec;10(12):6595–6610.
Journal cover image

Published In

Biomedical optics express

DOI

EISSN

2156-7085

ISSN

2156-7085

Publication Date

December 2019

Volume

10

Issue

12

Start / End Page

6595 / 6610

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
  • 3212 Ophthalmology and optometry
  • 0912 Materials Engineering
  • 0205 Optical Physics