Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.
Publication
, Journal Article
Korneev, MJ; Lakämper, S; Schmidt, CF
Published in: European biophysics journal : EBJ
July 2007
Tetrameric motor proteins of the Kinesin-5 family are essential for eukaryotic cell division. The microscopic mechanism by which Eg5, the vertebrate Kinesin-5, drives bipolar mitotic spindle formation remains unknown. Here we show in optical trapping experiments that full-length Eg5 moves processively and stepwise along microtubule bundles. Interestingly, the force produced by individual Eg5 motors typically reached only approximately 2 pN, one-third of the stall force of Kinesin-1. Eg5 typically detached from microtubules before stalling. This behavior may reflect a regulatory mechanism important for the role of Eg5 in the mitotic spindle.
Duke Scholars
Published In
European biophysics journal : EBJ
DOI
EISSN
1432-1017
ISSN
0175-7571
Publication Date
July 2007
Volume
36
Issue
6
Start / End Page
675 / 681
Related Subject Headings
- Xenopus laevis
- Xenopus Proteins
- Optical Tweezers
- Motion
- Microtubules
- Kinesins
- Biophysics
- Animals
- 5105 Medical and biological physics
- 3101 Biochemistry and cell biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Korneev, M. J., Lakämper, S., & Schmidt, C. F. (2007). Load-dependent release limits the processive stepping of the tetrameric Eg5 motor. European Biophysics Journal : EBJ, 36(6), 675–681. https://doi.org/10.1007/s00249-007-0134-6
Korneev, Mikhail J., Stefan Lakämper, and Christoph F. Schmidt. “Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.” European Biophysics Journal : EBJ 36, no. 6 (July 2007): 675–81. https://doi.org/10.1007/s00249-007-0134-6.
Korneev MJ, Lakämper S, Schmidt CF. Load-dependent release limits the processive stepping of the tetrameric Eg5 motor. European biophysics journal : EBJ. 2007 Jul;36(6):675–81.
Korneev, Mikhail J., et al. “Load-dependent release limits the processive stepping of the tetrameric Eg5 motor.” European Biophysics Journal : EBJ, vol. 36, no. 6, July 2007, pp. 675–81. Epmc, doi:10.1007/s00249-007-0134-6.
Korneev MJ, Lakämper S, Schmidt CF. Load-dependent release limits the processive stepping of the tetrameric Eg5 motor. European biophysics journal : EBJ. 2007 Jul;36(6):675–681.
Published In
European biophysics journal : EBJ
DOI
EISSN
1432-1017
ISSN
0175-7571
Publication Date
July 2007
Volume
36
Issue
6
Start / End Page
675 / 681
Related Subject Headings
- Xenopus laevis
- Xenopus Proteins
- Optical Tweezers
- Motion
- Microtubules
- Kinesins
- Biophysics
- Animals
- 5105 Medical and biological physics
- 3101 Biochemistry and cell biology