In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.
Publication
, Journal Article
Espinosa, JR; Wheaton, JD; Ciofani, M
Published in: Methods Mol Biol
2020
In vitro differentiation of naïve CD4+ T cells into effector and regulatory subsets offers a means to acquire large numbers of relatively homogeneous cell populations for experimentation. However, culture systems for T cell differentiation described in the literature vary widely in efficiency and complexity, limiting their comparison across studies. Here, we present a standardized and robust method for the isolation and in vitro differentiation of six CD4+ T cell subsets from mouse naïve T cells.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2020
Volume
2111
Start / End Page
79 / 89
Location
United States
Related Subject Headings
- T-Lymphocytes, Regulatory
- T-Lymphocyte Subsets
- Mice
- Lymphocyte Activation
- Developmental Biology
- Cells, Cultured
- Cell Polarity
- Cell Differentiation
- CD4-Positive T-Lymphocytes
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Espinosa, J. R., Wheaton, J. D., & Ciofani, M. (2020). In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets. Methods Mol Biol, 2111, 79–89. https://doi.org/10.1007/978-1-0716-0266-9_7
Espinosa, Jaclyn R., Joshua D. Wheaton, and Maria Ciofani. “In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.” Methods Mol Biol 2111 (2020): 79–89. https://doi.org/10.1007/978-1-0716-0266-9_7.
Espinosa JR, Wheaton JD, Ciofani M. In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets. Methods Mol Biol. 2020;2111:79–89.
Espinosa, Jaclyn R., et al. “In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets.” Methods Mol Biol, vol. 2111, 2020, pp. 79–89. Pubmed, doi:10.1007/978-1-0716-0266-9_7.
Espinosa JR, Wheaton JD, Ciofani M. In Vitro Differentiation of CD4+ T Cell Effector and Regulatory Subsets. Methods Mol Biol. 2020;2111:79–89.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2020
Volume
2111
Start / End Page
79 / 89
Location
United States
Related Subject Headings
- T-Lymphocytes, Regulatory
- T-Lymphocyte Subsets
- Mice
- Lymphocyte Activation
- Developmental Biology
- Cells, Cultured
- Cell Polarity
- Cell Differentiation
- CD4-Positive T-Lymphocytes
- Animals