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Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis.

Publication ,  Journal Article
Ganley, JG; D'Ambrosio, HK; Shieh, M; Derbyshire, ER
Published in: Chembiochem : a European journal of chemical biology
May 2020

Anopheles mosquito microbiomes are intriguing ecological niches. Within the gut, microbes adapt to oxidative stress due to heme and iron after blood meals. Although metagenomic sequencing has illuminated spatial and temporal fluxes of microbiome populations, limited data exist on microbial growth dynamics. Here, we analyze growth interactions between a dominant microbiome species, Elizabethkingia anophelis, and other Anopheles-associated bacteria. We find E. anophelis inhibits a Pseudomonas sp. via an antimicrobial-independent mechanism and observe biliverdins, heme degradation products, upregulated in cocultures. Purification and characterization of E. anophelis HemS demonstrates heme degradation, and we observe hemS expression is upregulated when cocultured with Pseudomonas sp. This study reveals a competitive microbial interaction between mosquito-associated bacteria and characterizes the stimulation of heme degradation in E. anophelis when grown with Pseudomonas sp.

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Published In

Chembiochem : a European journal of chemical biology

DOI

EISSN

1439-7633

ISSN

1439-4227

Publication Date

May 2020

Volume

21

Issue

9

Start / End Page

1279 / 1284

Related Subject Headings

  • Virulence
  • Sequence Analysis, DNA
  • Phylogeny
  • Organic Chemistry
  • Microbiota
  • Heme
  • Genome, Bacterial
  • Flavobacteriaceae
  • Coculture Techniques
  • Bacterial Proteins
 

Citation

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Ganley, J. G., D’Ambrosio, H. K., Shieh, M., & Derbyshire, E. R. (2020). Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis. Chembiochem : A European Journal of Chemical Biology, 21(9), 1279–1284. https://doi.org/10.1002/cbic.201900675
Ganley, Jack G., Hannah K. D’Ambrosio, Meg Shieh, and Emily R. Derbyshire. “Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis.Chembiochem : A European Journal of Chemical Biology 21, no. 9 (May 2020): 1279–84. https://doi.org/10.1002/cbic.201900675.
Ganley JG, D’Ambrosio HK, Shieh M, Derbyshire ER. Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis. Chembiochem : a European journal of chemical biology. 2020 May;21(9):1279–84.
Ganley, Jack G., et al. “Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis.Chembiochem : A European Journal of Chemical Biology, vol. 21, no. 9, May 2020, pp. 1279–84. Epmc, doi:10.1002/cbic.201900675.
Ganley JG, D’Ambrosio HK, Shieh M, Derbyshire ER. Coculturing of Mosquito-Microbiome Bacteria Promotes Heme Degradation in Elizabethkingia anophelis. Chembiochem : a European journal of chemical biology. 2020 May;21(9):1279–1284.
Journal cover image

Published In

Chembiochem : a European journal of chemical biology

DOI

EISSN

1439-7633

ISSN

1439-4227

Publication Date

May 2020

Volume

21

Issue

9

Start / End Page

1279 / 1284

Related Subject Headings

  • Virulence
  • Sequence Analysis, DNA
  • Phylogeny
  • Organic Chemistry
  • Microbiota
  • Heme
  • Genome, Bacterial
  • Flavobacteriaceae
  • Coculture Techniques
  • Bacterial Proteins