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De novo engineering of intracellular condensates using artificial disordered proteins.

Publication ,  Journal Article
Dzuricky, M; Rogers, BA; Shahid, A; Cremer, PS; Chilkoti, A
Published in: Nature chemistry
September 2020

Phase separation of intrinsically disordered proteins (IDPs) is a remarkable feature of living cells to dynamically control intracellular partitioning. Despite the numerous new IDPs that have been identified, progress towards rational engineering in cells has been limited. To address this limitation, we systematically scanned the sequence space of native IDPs and designed artificial IDPs (A-IDPs) with different molecular weights and aromatic content, which exhibit variable condensate saturation concentrations and temperature cloud points in vitro and in cells. We created A-IDP puncta using these simple principles, which are capable of sequestering an enzyme and whose catalytic efficiency can be manipulated by the molecular weight of the A-IDP. These results provide a robust engineered platform for creating puncta with new, phase-separation-mediated control of biological function in living cells.

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Published In

Nature chemistry

DOI

EISSN

1755-4349

ISSN

1755-4330

Publication Date

September 2020

Volume

12

Issue

9

Start / End Page

814 / 825

Related Subject Headings

  • Transition Temperature
  • Recombinant Proteins
  • Proteomics
  • Organic Chemistry
  • Mutagenesis
  • Molecular Weight
  • Kinetics
  • Intrinsically Disordered Proteins
  • Humans
  • Escherichia coli
 

Citation

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Dzuricky, M., Rogers, B. A., Shahid, A., Cremer, P. S., & Chilkoti, A. (2020). De novo engineering of intracellular condensates using artificial disordered proteins. Nature Chemistry, 12(9), 814–825. https://doi.org/10.1038/s41557-020-0511-7
Dzuricky, Michael, Bradley A. Rogers, Abdulla Shahid, Paul S. Cremer, and Ashutosh Chilkoti. “De novo engineering of intracellular condensates using artificial disordered proteins.Nature Chemistry 12, no. 9 (September 2020): 814–25. https://doi.org/10.1038/s41557-020-0511-7.
Dzuricky M, Rogers BA, Shahid A, Cremer PS, Chilkoti A. De novo engineering of intracellular condensates using artificial disordered proteins. Nature chemistry. 2020 Sep;12(9):814–25.
Dzuricky, Michael, et al. “De novo engineering of intracellular condensates using artificial disordered proteins.Nature Chemistry, vol. 12, no. 9, Sept. 2020, pp. 814–25. Epmc, doi:10.1038/s41557-020-0511-7.
Dzuricky M, Rogers BA, Shahid A, Cremer PS, Chilkoti A. De novo engineering of intracellular condensates using artificial disordered proteins. Nature chemistry. 2020 Sep;12(9):814–825.

Published In

Nature chemistry

DOI

EISSN

1755-4349

ISSN

1755-4330

Publication Date

September 2020

Volume

12

Issue

9

Start / End Page

814 / 825

Related Subject Headings

  • Transition Temperature
  • Recombinant Proteins
  • Proteomics
  • Organic Chemistry
  • Mutagenesis
  • Molecular Weight
  • Kinetics
  • Intrinsically Disordered Proteins
  • Humans
  • Escherichia coli