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Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils.

Publication ,  Journal Article
Jang, LK; Alvarado, JA; Pepona, M; Wasson, EM; Nash, LD; Ortega, JM; Randles, A; Maitland, DJ; Moya, ML; Hynes, WF
Published in: Biofabrication
October 2020

Various types of embolization devices have been developed for the treatment of cerebral aneurysms. However, it is challenging to properly evaluate device performance and train medical personnel for device deployment without the aid of functionally relevant models. Currentin vitroaneurysm models suffer from a lack of key functional and morphological features of brain vasculature that limit their applicability for these purposes. These features include the physiologically relevant mechanical properties and the dynamic cellular environment of blood vessels subjected to constant fluid flow. Herein, we developed three-dimensionally (3D) printed aneurysm-bearing vascularized tissue structures using gelatin-fibrin hydrogel of which the inner vessel walls were seeded with human cerebral microvascular endothelial cells (hCMECs). The hCMECs readily exhibited cellular attachment, spreading, and confluency all around the vessel walls, including the aneurysm walls. Additionally, thein vitroplatform was directly amenable to flow measurements via particle image velocimetry, enabling the direct assessment of the vascular flow dynamics for comparison to a 3D computational fluid dynamics model. Detachable coils were delivered into the printed aneurysm sac through the vessel using a microcatheter and static blood plasma clotting was monitored inside the aneurysm sac and around the coils. This biomimeticin vitroaneurysm model is a promising method for examining the biocompatibility and hemostatic efficiency of embolization devices and for providing hemodynamic information which would aid in predicting aneurysm rupture or healing response after treatment.

Duke Scholars

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Published In

Biofabrication

DOI

EISSN

1758-5090

ISSN

1758-5082

Publication Date

October 2020

Volume

13

Issue

1

Related Subject Headings

  • Intracranial Aneurysm
  • Humans
  • Endothelial Cells
  • Embolization, Therapeutic
  • Blood Vessel Prosthesis
  • Bioprinting
  • 4003 Biomedical engineering
  • 3206 Medical biotechnology
  • 1099 Other Technology
  • 1004 Medical Biotechnology
 

Citation

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Jang, L. K., Alvarado, J. A., Pepona, M., Wasson, E. M., Nash, L. D., Ortega, J. M., … Hynes, W. F. (2020). Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils. Biofabrication, 13(1). https://doi.org/10.1088/1758-5090/abbb9b
Jang, Lindy K., Javier A. Alvarado, Marianna Pepona, Elisa M. Wasson, Landon D. Nash, Jason M. Ortega, Amanda Randles, Duncan J. Maitland, Monica L. Moya, and William F. Hynes. “Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils.Biofabrication 13, no. 1 (October 2020). https://doi.org/10.1088/1758-5090/abbb9b.
Jang LK, Alvarado JA, Pepona M, Wasson EM, Nash LD, Ortega JM, et al. Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils. Biofabrication. 2020 Oct;13(1).
Jang, Lindy K., et al. “Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils.Biofabrication, vol. 13, no. 1, Oct. 2020. Epmc, doi:10.1088/1758-5090/abbb9b.
Jang LK, Alvarado JA, Pepona M, Wasson EM, Nash LD, Ortega JM, Randles A, Maitland DJ, Moya ML, Hynes WF. Three-dimensional bioprinting of aneurysm-bearing tissue structure for endovascular deployment of embolization coils. Biofabrication. 2020 Oct;13(1).
Journal cover image

Published In

Biofabrication

DOI

EISSN

1758-5090

ISSN

1758-5082

Publication Date

October 2020

Volume

13

Issue

1

Related Subject Headings

  • Intracranial Aneurysm
  • Humans
  • Endothelial Cells
  • Embolization, Therapeutic
  • Blood Vessel Prosthesis
  • Bioprinting
  • 4003 Biomedical engineering
  • 3206 Medical biotechnology
  • 1099 Other Technology
  • 1004 Medical Biotechnology