An Improved Protocol for the Production of Lentiviral Vectors.
Publication
, Journal Article
Brown, LY; Dong, W; Kantor, B
Published in: STAR Protoc
December 18, 2020
Lentiviral vectors are an ideal gene-delivery system for large gene-editing tools, such as the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system, due to their high packaging capacity and broad tropism. Here, we present a calcium phosphate-based protocol for lentiviral production and concentration for in vitro and in vivo use. This revised procedure has been optimized to ensure high viral titers and transduction efficiency and is scalable to meet specific production needs.
Duke Scholars
Published In
STAR Protoc
DOI
EISSN
2666-1667
Publication Date
December 18, 2020
Volume
1
Issue
3
Start / End Page
100152
Location
United States
Related Subject Headings
- Virion
- Ultracentrifugation
- Transfection
- Plasmids
- Lentivirus
- Humans
- HEK293 Cells
- Genetic Vectors
- Genetic Techniques
Citation
APA
Chicago
ICMJE
MLA
NLM
Brown, L. Y., Dong, W., & Kantor, B. (2020). An Improved Protocol for the Production of Lentiviral Vectors. STAR Protoc, 1(3), 100152. https://doi.org/10.1016/j.xpro.2020.100152
Brown, Logan Y., Wendy Dong, and Boris Kantor. “An Improved Protocol for the Production of Lentiviral Vectors.” STAR Protoc 1, no. 3 (December 18, 2020): 100152. https://doi.org/10.1016/j.xpro.2020.100152.
Brown LY, Dong W, Kantor B. An Improved Protocol for the Production of Lentiviral Vectors. STAR Protoc. 2020 Dec 18;1(3):100152.
Brown, Logan Y., et al. “An Improved Protocol for the Production of Lentiviral Vectors.” STAR Protoc, vol. 1, no. 3, Dec. 2020, p. 100152. Pubmed, doi:10.1016/j.xpro.2020.100152.
Brown LY, Dong W, Kantor B. An Improved Protocol for the Production of Lentiviral Vectors. STAR Protoc. 2020 Dec 18;1(3):100152.
Published In
STAR Protoc
DOI
EISSN
2666-1667
Publication Date
December 18, 2020
Volume
1
Issue
3
Start / End Page
100152
Location
United States
Related Subject Headings
- Virion
- Ultracentrifugation
- Transfection
- Plasmids
- Lentivirus
- Humans
- HEK293 Cells
- Genetic Vectors
- Genetic Techniques