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Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells.

Publication ,  Journal Article
Gupta, RK; Mepani, RJ; Kleiner, S; Lo, JC; Khandekar, MJ; Cohen, P; Frontini, A; Bhowmick, DC; Ye, L; Cinti, S; Spiegelman, BM
Published in: Cell Metab
February 8, 2012

Progress has been made in elucidating the cell-surface phenotype of primary adipose progenitors; however, specific functional markers and distinct molecular signatures of fat depot-specific preadipocytes have remained elusive. In this study, we label committed murine adipose progenitors through expression of GFP from the genetic locus for Zfp423, a gene controlling preadipocyte determination. Selection of GFP-expressing fibroblasts from either subcutaneous or visceral adipose-derived stromal vascular cultures isolates stably committed preadipocytes that undergo robust adipogenesis. Immunohistochemistry for Zfp423-driven GFP expression in vivo confirms a perivascular origin of preadipocytes within both white and brown adipose tissues. Interestingly, a small subset of capillary endothelial cells within white and brown fat also express this marker, suggesting a contribution of specialized endothelial cells to the adipose lineage. Zfp423(GFP) mice represent a simple tool for the specific localization and isolation of molecularly defined preadipocytes from distinct adipose tissue depots.

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Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

February 8, 2012

Volume

15

Issue

2

Start / End Page

230 / 239

Location

United States

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Pericytes
  • Mice, Transgenic
  • Mice
  • Immunohistochemistry
  • Green Fluorescent Proteins
  • Gene Expression Profiling
  • Flow Cytometry
  • Endothelial Cells
 

Citation

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Chicago
ICMJE
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Gupta, R. K., Mepani, R. J., Kleiner, S., Lo, J. C., Khandekar, M. J., Cohen, P., … Spiegelman, B. M. (2012). Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. Cell Metab, 15(2), 230–239. https://doi.org/10.1016/j.cmet.2012.01.010
Gupta, Rana K., Rina J. Mepani, Sandra Kleiner, James C. Lo, Melin J. Khandekar, Paul Cohen, Andrea Frontini, et al. “Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells.Cell Metab 15, no. 2 (February 8, 2012): 230–39. https://doi.org/10.1016/j.cmet.2012.01.010.
Gupta RK, Mepani RJ, Kleiner S, Lo JC, Khandekar MJ, Cohen P, et al. Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. Cell Metab. 2012 Feb 8;15(2):230–9.
Gupta, Rana K., et al. “Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells.Cell Metab, vol. 15, no. 2, Feb. 2012, pp. 230–39. Pubmed, doi:10.1016/j.cmet.2012.01.010.
Gupta RK, Mepani RJ, Kleiner S, Lo JC, Khandekar MJ, Cohen P, Frontini A, Bhowmick DC, Ye L, Cinti S, Spiegelman BM. Zfp423 expression identifies committed preadipocytes and localizes to adipose endothelial and perivascular cells. Cell Metab. 2012 Feb 8;15(2):230–239.
Journal cover image

Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

February 8, 2012

Volume

15

Issue

2

Start / End Page

230 / 239

Location

United States

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Pericytes
  • Mice, Transgenic
  • Mice
  • Immunohistochemistry
  • Green Fluorescent Proteins
  • Gene Expression Profiling
  • Flow Cytometry
  • Endothelial Cells