Skip to main content

A single-cell based precision medicine approach using glioblastoma patient-specific models.

Publication ,  Journal Article
Park, JH; Feroze, AH; Emerson, SN; Mihalas, AB; Keene, CD; Cimino, PJ; de Lomana, ALG; Kannan, K; Wu, W-J; Turkarslan, S; Baliga, NS; Patel, AP
Published in: NPJ Precis Oncol
August 8, 2022

Glioblastoma (GBM) is a heterogeneous tumor made up of cell states that evolve over time. Here, we modeled tumor evolutionary trajectories during standard-of-care treatment using multi-omic single-cell analysis of a primary tumor sample, corresponding mouse xenografts subjected to standard of care therapy, and recurrent tumor at autopsy. We mined the multi-omic data with single-cell SYstems Genetics Network AnaLysis (scSYGNAL) to identify a network of 52 regulators that mediate treatment-induced shifts in xenograft tumor-cell states that were also reflected in recurrence. By integrating scSYGNAL-derived regulatory network information with transcription factor accessibility deviations derived from single-cell ATAC-seq data, we developed consensus networks that modulate cell state transitions across subpopulations of primary and recurrent tumor cells. Finally, by matching targeted therapies to active regulatory networks underlying tumor evolutionary trajectories, we provide a framework for applying single-cell-based precision medicine approaches to an individual patient in a concurrent, adjuvant, or recurrent setting.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

NPJ Precis Oncol

DOI

ISSN

2397-768X

Publication Date

August 8, 2022

Volume

6

Issue

1

Start / End Page

55

Location

England

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 3204 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Park, J. H., Feroze, A. H., Emerson, S. N., Mihalas, A. B., Keene, C. D., Cimino, P. J., … Patel, A. P. (2022). A single-cell based precision medicine approach using glioblastoma patient-specific models. NPJ Precis Oncol, 6(1), 55. https://doi.org/10.1038/s41698-022-00294-4
Park, James H., Abdullah H. Feroze, Samuel N. Emerson, Anca B. Mihalas, C Dirk Keene, Patrick J. Cimino, Adrian Lopez Garcia de Lomana, et al. “A single-cell based precision medicine approach using glioblastoma patient-specific models.NPJ Precis Oncol 6, no. 1 (August 8, 2022): 55. https://doi.org/10.1038/s41698-022-00294-4.
Park JH, Feroze AH, Emerson SN, Mihalas AB, Keene CD, Cimino PJ, et al. A single-cell based precision medicine approach using glioblastoma patient-specific models. NPJ Precis Oncol. 2022 Aug 8;6(1):55.
Park, James H., et al. “A single-cell based precision medicine approach using glioblastoma patient-specific models.NPJ Precis Oncol, vol. 6, no. 1, Aug. 2022, p. 55. Pubmed, doi:10.1038/s41698-022-00294-4.
Park JH, Feroze AH, Emerson SN, Mihalas AB, Keene CD, Cimino PJ, de Lomana ALG, Kannan K, Wu W-J, Turkarslan S, Baliga NS, Patel AP. A single-cell based precision medicine approach using glioblastoma patient-specific models. NPJ Precis Oncol. 2022 Aug 8;6(1):55.

Published In

NPJ Precis Oncol

DOI

ISSN

2397-768X

Publication Date

August 8, 2022

Volume

6

Issue

1

Start / End Page

55

Location

England

Related Subject Headings

  • 3211 Oncology and carcinogenesis
  • 3204 Immunology