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Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes.

Publication ,  Journal Article
Balsinde, J; Barbour, SE; Bianco, ID; Dennis, EA
Published in: Proc Natl Acad Sci U S A
November 8, 1994

Macrophage-like P388D1 cells mobilize arachidonic acid (AA) and produce prostaglandin E2 upon stimulation with bacterial lipopolysaccharide and platelet-activating factor. We have now demonstrated that AA mobilization in these cells is composed of two distinct events: a transient phase in which AA accumulates in the cell and a sustained phase in which the fatty acid accumulates in the incubation medium. Both phases are markedly dependent on the presence of Ca2+ in the extracellular medium. Treatment with an antisense oligonucleotide to group II phospholipase A2 inhibits the accumulation of AA in the incubation medium, but has no effect on the accumulation of this fatty acid in the cell. In addition, treatment with antisense oligonucleotide to group II phospholipase A2 has no effect on the uptake or the esterification of AA. Collectively, these results indicate that, in addition to the previously demonstrated role of group II phospholipase A2 in AA mobilization in activated P388D1 cells, another phospholipase A2, distinct from the group II enzyme, is implicated in raising the levels of intracellular AA during the early steps of P388D1 cell activation and in modulating deacylation/reacylation reactions involving AA. The data suggest that each of the different phospholipase A2 enzymes present in P388D1 cells serves a distinct role in cell function.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11060 / 11064

Location

United States

Related Subject Headings

  • Time Factors
  • RNA, Messenger
  • Platelet Activating Factor
  • Phospholipids
  • Phospholipases A2
  • Phospholipases A
  • Molecular Sequence Data
  • Mice
  • Membrane Lipids
  • Macrophages
 

Citation

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Balsinde, J., Barbour, S. E., Bianco, I. D., & Dennis, E. A. (1994). Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes. Proc Natl Acad Sci U S A, 91(23), 11060–11064. https://doi.org/10.1073/pnas.91.23.11060
Balsinde, J., S. E. Barbour, I. D. Bianco, and E. A. Dennis. “Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes.Proc Natl Acad Sci U S A 91, no. 23 (November 8, 1994): 11060–64. https://doi.org/10.1073/pnas.91.23.11060.
Balsinde J, Barbour SE, Bianco ID, Dennis EA. Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11060–4.
Balsinde, J., et al. “Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes.Proc Natl Acad Sci U S A, vol. 91, no. 23, Nov. 1994, pp. 11060–64. Pubmed, doi:10.1073/pnas.91.23.11060.
Balsinde J, Barbour SE, Bianco ID, Dennis EA. Arachidonic acid mobilization in P388D1 macrophages is controlled by two distinct Ca(2+)-dependent phospholipase A2 enzymes. Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11060–11064.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

November 8, 1994

Volume

91

Issue

23

Start / End Page

11060 / 11064

Location

United States

Related Subject Headings

  • Time Factors
  • RNA, Messenger
  • Platelet Activating Factor
  • Phospholipids
  • Phospholipases A2
  • Phospholipases A
  • Molecular Sequence Data
  • Mice
  • Membrane Lipids
  • Macrophages