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In vitro reconstitution of septin assemblies on supported lipid bilayers.

Publication ,  Journal Article
Bridges, AA; Gladfelter, AS
Published in: Methods Cell Biol
2016

Septins are polymerizing eukaryotic proteins that play conserved roles in cell cortex organization and are essential in many cell types. How septin dynamics and protein-protein interactions determine their function at the plasma membrane remains a mystery. Here, we present a method for recapitulating septin polymerization and lipid interaction utilizing supported lipid bilayers to mimic the eukaryotic plasma membrane. Septins on supported lipid bilayers can be visualized with single-molecule sensitivity using total internal reflective fluorescence microscopy. Microscopy-based in vitro assays have revolutionized our understanding of actin, microtubules, and bacterial cytoskeletal systems, and will likely immediately advance our understanding of the septin proteins. As such, we hope that this technique will be adopted and widely utilized by those interested in uncovering septin properties and functions of septin interacting proteins.

Duke Scholars

Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2016

Volume

136

Start / End Page

57 / 71

Location

United States

Related Subject Headings

  • Septins
  • Protein Multimerization
  • Protein Interaction Maps
  • Microtubules
  • Microscopy
  • Lipid Bilayers
  • Developmental Biology
  • Cytoskeleton
  • Cytoplasm
  • Cell Membrane
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bridges, A. A., & Gladfelter, A. S. (2016). In vitro reconstitution of septin assemblies on supported lipid bilayers. Methods Cell Biol, 136, 57–71. https://doi.org/10.1016/bs.mcb.2016.03.025
Bridges, A. A., and A. S. Gladfelter. “In vitro reconstitution of septin assemblies on supported lipid bilayers.Methods Cell Biol 136 (2016): 57–71. https://doi.org/10.1016/bs.mcb.2016.03.025.
Bridges AA, Gladfelter AS. In vitro reconstitution of septin assemblies on supported lipid bilayers. Methods Cell Biol. 2016;136:57–71.
Bridges, A. A., and A. S. Gladfelter. “In vitro reconstitution of septin assemblies on supported lipid bilayers.Methods Cell Biol, vol. 136, 2016, pp. 57–71. Pubmed, doi:10.1016/bs.mcb.2016.03.025.
Bridges AA, Gladfelter AS. In vitro reconstitution of septin assemblies on supported lipid bilayers. Methods Cell Biol. 2016;136:57–71.
Journal cover image

Published In

Methods Cell Biol

DOI

ISSN

0091-679X

Publication Date

2016

Volume

136

Start / End Page

57 / 71

Location

United States

Related Subject Headings

  • Septins
  • Protein Multimerization
  • Protein Interaction Maps
  • Microtubules
  • Microscopy
  • Lipid Bilayers
  • Developmental Biology
  • Cytoskeleton
  • Cytoplasm
  • Cell Membrane