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Asynchronous nuclear division cycles in multinucleated cells.

Publication ,  Journal Article
Gladfelter, AS; Hungerbuehler, AK; Philippsen, P
Published in: J Cell Biol
January 30, 2006

Synchronous mitosis is common in multinucleated cells. We analyzed a unique asynchronous nuclear division cycle in a multinucleated filamentous fungus, Ashbya gossypii. Nuclear pedigree analysis and observation of GFP-labeled spindle pole bodies demonstrated that neighboring nuclei in A. gossypii cells are in different cell cycle stages despite close physical proximity. Neighboring nuclei did not differ significantly in their patterns of cyclin protein localization such that both G1 and mitotic cyclins were present regardless of cell cycle stage, suggesting that the complete destruction of cyclins is not occurring in this system. Indeed, the expression of mitotic cyclin lacking NH(2)-terminal destruction box sequences did not block cell cycle progression. Cells lacking AgSic1p, a predicted cyclin-dependent kinase (CDK) inhibitor, however, showed aberrant multipolar spindles and fragmented nuclei that are indicative of flawed mitoses. We hypothesize that the continuous cytoplasm in these cells promoted the evolution of a nuclear division cycle in which CDK inhibitors primarily control CDK activity rather than oscillating mitotic cyclin proteins.

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Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

January 30, 2006

Volume

172

Issue

3

Start / End Page

347 / 362

Location

United States

Related Subject Headings

  • Spindle Apparatus
  • Sequence Homology, Amino Acid
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Protein-Tyrosine Kinases
  • Nuclear Export Signals
  • Mutation
  • Mitosis
  • Histones
  • Fungal Proteins
 

Citation

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Gladfelter, A. S., Hungerbuehler, A. K., & Philippsen, P. (2006). Asynchronous nuclear division cycles in multinucleated cells. J Cell Biol, 172(3), 347–362. https://doi.org/10.1083/jcb.200507003
Gladfelter, Amy S., A Katrin Hungerbuehler, and Peter Philippsen. “Asynchronous nuclear division cycles in multinucleated cells.J Cell Biol 172, no. 3 (January 30, 2006): 347–62. https://doi.org/10.1083/jcb.200507003.
Gladfelter AS, Hungerbuehler AK, Philippsen P. Asynchronous nuclear division cycles in multinucleated cells. J Cell Biol. 2006 Jan 30;172(3):347–62.
Gladfelter, Amy S., et al. “Asynchronous nuclear division cycles in multinucleated cells.J Cell Biol, vol. 172, no. 3, Jan. 2006, pp. 347–62. Pubmed, doi:10.1083/jcb.200507003.
Gladfelter AS, Hungerbuehler AK, Philippsen P. Asynchronous nuclear division cycles in multinucleated cells. J Cell Biol. 2006 Jan 30;172(3):347–362.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

January 30, 2006

Volume

172

Issue

3

Start / End Page

347 / 362

Location

United States

Related Subject Headings

  • Spindle Apparatus
  • Sequence Homology, Amino Acid
  • Saccharomycetales
  • Saccharomyces cerevisiae Proteins
  • Protein-Tyrosine Kinases
  • Nuclear Export Signals
  • Mutation
  • Mitosis
  • Histones
  • Fungal Proteins