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Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.

Publication ,  Journal Article
Rayens, NT; Cook, KJ; McKinley, SA; Payne, CK
Published in: Biophysical journal
April 2023

Essential cellular processes such as metabolism, protein synthesis, and autophagy require the intracellular transport of membrane-bound vesicles. The importance of the cytoskeleton and associated molecular motors for transport is well documented. Recent research has suggested that the endoplasmic reticulum (ER) may also play a role in vesicle transport through a tethering of vesicles to the ER. We use single-particle tracking fluorescence microscopy and a Bayesian change-point algorithm to characterize vesicle motility in response to the disruption of the ER, actin, and microtubules. This high-throughput change-point algorithm allows us to efficiently analyze thousands of trajectory segments. We find that palmitate-mediated disruption of the ER leads to a significant decrease in vesicle motility. A comparison with the disruption of actin and microtubules shows that disruption of the ER has a significant impact on vesicle motility, greater than the disruption of actin. Vesicle motility was dependent on cellular region, with greater motility in the cell periphery than the perinuclear region, possibly due to regional differences in actin and the ER. Overall, these results suggest that the ER is an important factor in vesicle transport.

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Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2023

Volume

122

Issue

7

Start / End Page

1355 / 1363

Related Subject Headings

  • Microtubules
  • Endoplasmic Reticulum
  • Cytoskeleton
  • Biophysics
  • Bayes Theorem
  • Actins
  • 51 Physical sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
 

Citation

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Rayens, N. T., Cook, K. J., McKinley, S. A., & Payne, C. K. (2023). Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility. Biophysical Journal, 122(7), 1355–1363. https://doi.org/10.1016/j.bpj.2023.03.001
Rayens, Nathan T., Keisha J. Cook, Scott A. McKinley, and Christine K. Payne. “Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.Biophysical Journal 122, no. 7 (April 2023): 1355–63. https://doi.org/10.1016/j.bpj.2023.03.001.
Rayens NT, Cook KJ, McKinley SA, Payne CK. Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility. Biophysical journal. 2023 Apr;122(7):1355–63.
Rayens, Nathan T., et al. “Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility.Biophysical Journal, vol. 122, no. 7, Apr. 2023, pp. 1355–63. Epmc, doi:10.1016/j.bpj.2023.03.001.
Rayens NT, Cook KJ, McKinley SA, Payne CK. Palmitate-mediated disruption of the endoplasmic reticulum decreases intracellular vesicle motility. Biophysical journal. 2023 Apr;122(7):1355–1363.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

April 2023

Volume

122

Issue

7

Start / End Page

1355 / 1363

Related Subject Headings

  • Microtubules
  • Endoplasmic Reticulum
  • Cytoskeleton
  • Biophysics
  • Bayes Theorem
  • Actins
  • 51 Physical sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences