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A physical sciences network characterization of non-tumorigenic and metastatic cells.

Publication ,  Journal Article
Physical Sciences - Oncology Centers Network; Agus, DB; Alexander, JF; Arap, W; Ashili, S; Aslan, JE; Austin, RH; Backman, V; Bethel, KJ; Foo, J ...
Published in: Scientific reports
January 2013
Published version (DOI)

To investigate the transition from non-cancerous to metastatic from a physical sciences perspective, the Physical Sciences-Oncology Centers (PS-OC) Network performed molecular and biophysical comparative studies of the non-tumorigenic MCF-10A and metastatic MDA-MB-231 breast epithelial cell lines, commonly used as models of cancer metastasis. Experiments were performed in 20 laboratories from 12 PS-OCs. Each laboratory was supplied with identical aliquots and common reagents and culture protocols. Analyses of these measurements revealed dramatic differences in their mechanics, migration, adhesion, oxygen response, and proteomic profiles. Model-based multi-omics approaches identified key differences between these cells' regulatory networks involved in morphology and survival. These results provide a multifaceted description of cellular parameters of two widely used cell lines and demonstrate the value of the PS-OC Network approach for integration of diverse experimental observations to elucidate the phenotypes associated with cancer metastasis.

Duke Scholars

Sharon Gerecht
Author Sharon Gerecht Biomedical Engineering
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Published In

Scientific reports

DOI

10.1038/srep01449

EISSN

2045-2322

ISSN

2045-2322

Publication Date

January 2013

Volume

3

Start / End Page

1449

Related Subject Headings

  • Neoplasm Proteins
  • Neoplasm Metastasis
  • Models, Biological
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Computer Simulation
  • Cell Survival
  • Cell Size
  • Cell Movement
  • Cell Line, Tumor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Physical Sciences - Oncology Centers Network, Agus, D. B., Alexander, J. F., Arap, W., Ashili, S., Aslan, J. E., … Wu, P.-H. (2013). A physical sciences network characterization of non-tumorigenic and metastatic cells. Scientific Reports, 3, 1449. https://doi.org/10.1038/srep01449
Physical Sciences - Oncology Centers Network, David B. Agus, Jenolyn F. Alexander, Wadih Arap, Shashanka Ashili, Joseph E. Aslan, Robert H. Austin, et al. “A physical sciences network characterization of non-tumorigenic and metastatic cells.” Scientific Reports 3 (January 2013): 1449. https://doi.org/10.1038/srep01449.
Physical Sciences - Oncology Centers Network, Agus DB, Alexander JF, Arap W, Ashili S, Aslan JE, et al. A physical sciences network characterization of non-tumorigenic and metastatic cells. Scientific reports. 2013 Jan;3:1449.
Physical Sciences - Oncology Centers Network, et al. “A physical sciences network characterization of non-tumorigenic and metastatic cells.” Scientific Reports, vol. 3, Jan. 2013, p. 1449. Epmc, doi:10.1038/srep01449.
Physical Sciences - Oncology Centers Network, Agus DB, Alexander JF, Arap W, Ashili S, Aslan JE, Austin RH, Backman V, Bethel KJ, Bonneau R, Chen W-C, Chen-Tanyolac C, Choi NC, Curley SA, Dallas M, Damania D, Davies PCW, Decuzzi P, Dickinson L, Estevez-Salmeron L, Estrella V, Ferrari M, Fischbach C, Foo J, Fraley SI, Frantz C, Fuhrmann A, Gascard P, Gatenby RA, Geng Y, Gerecht S, Gillies RJ, Godin B, Grady WM, Greenfield A, Hemphill C, Hempstead BL, Hielscher A, Hillis WD, Holland EC, Ibrahim-Hashim A, Jacks T, Johnson RH, Joo A, Katz JE, Kelbauskas L, Kesselman C, King MR, Konstantopoulos K, Kraning-Rush CM, Kuhn P, Kung K, Kwee B, Lakins JN, Lambert G, Liao D, Licht JD, Liphardt JT, Liu L, Lloyd MC, Lyubimova A, Mallick P, Marko J, McCarty OJT, Meldrum DR, Michor F, Mumenthaler SM, Nandakumar V, O’Halloran TV, Oh S, Pasqualini R, Paszek MJ, Philips KG, Poultney CS, Rana K, Reinhart-King CA, Ros R, Semenza GL, Senechal P, Shuler ML, Srinivasan S, Staunton JR, Stypula Y, Subramanian H, Tlsty TD, Tormoen GW, Tseng Y, van Oudenaarden A, Verbridge SS, Wan JC, Weaver VM, Widom J, Will C, Wirtz D, Wojtkowiak J, Wu P-H. A physical sciences network characterization of non-tumorigenic and metastatic cells. Scientific reports. 2013 Jan;3:1449.

Published In

Scientific reports

DOI

10.1038/srep01449

EISSN

2045-2322

ISSN

2045-2322

Publication Date

January 2013

Volume

3

Start / End Page

1449

Related Subject Headings

  • Neoplasm Proteins
  • Neoplasm Metastasis
  • Models, Biological
  • Humans
  • Gene Expression Regulation, Neoplastic
  • Computer Simulation
  • Cell Survival
  • Cell Size
  • Cell Movement
  • Cell Line, Tumor