High-throughput imaging across multiple spatial scales with the multi-camera array microscope (MCAM)

“Anyone who uses a microscope has likely noticed the limitation of the trade-off between the field of view and the resolution”.1 To acquire highly resolved images at large fields of view, existing techniques typically record sequential images at different positions and then digitally stitch composite images. There are alternatives to this mechanical scanning procedure, such as structured illumination microscopy2 or Fourier ptychography3,4 that record sequential images at varying illuminations prevent mechanical scanning for high-resolution image composites. However, all of these approaches require sequential images and thus compromise speed, temporal resolution and experimental throughput. Here we present the Multi-Camera Array Microscope (MCAM), which is a microscope system that utilizes an array of many synchronized cameras, each with an individual imaging lens, for simultaneous image capture. The MCAM enables enhanced imaging capabilities and novel applications in various scientific and medical fields, by combining the images acquired from each individual camera-lens pair.

Duke Scholars

Author Roarke Horstmeyer Biomedical Engineering