Direct characterization of solid phase resin-bound molecules by mass spectrometry
Publication
, Journal Article
Fitzgerald, MC; Harris, K; Shevlin, CG; Siuzdak, G
Published in: Bioorganic and Medicinal Chemistry Letters
April 23, 1996
In this work we demonstrate that analytes, covalently linked to a polymeric support through a photolabile linker, can be directly analyzed by matrix-assisted laser desorption/ionization (MALDI). Mass spectral analysis is performed in a single step requiring no pretreatment of the sample to induce cleavage from the support. Our results show that the UV laser light in the MALDI experiment can be used to simultaneously promote an analyte's photolytic cleavage from a solid support and its gas phase ionization for subsequent mass spectral analysis. In this manner, MALDI facilitates the dissociation and identification of resin-bound analytes in a single analytical procedure without the need for any prior chemical treatment.
Duke Scholars
Published In
Bioorganic and Medicinal Chemistry Letters
DOI
ISSN
0960-894X
Publication Date
April 23, 1996
Volume
6
Issue
8
Start / End Page
979 / 982
Related Subject Headings
- Medicinal & Biomolecular Chemistry
- 3405 Organic chemistry
- 3404 Medicinal and biomolecular chemistry
- 1115 Pharmacology and Pharmaceutical Sciences
- 0305 Organic Chemistry
- 0304 Medicinal and Biomolecular Chemistry
Citation
APA
Chicago
ICMJE
MLA
NLM
Fitzgerald, M. C., Harris, K., Shevlin, C. G., & Siuzdak, G. (1996). Direct characterization of solid phase resin-bound molecules by mass spectrometry. Bioorganic and Medicinal Chemistry Letters, 6(8), 979–982. https://doi.org/10.1016/0960-894X(96)00152-7
Fitzgerald, M. C., K. Harris, C. G. Shevlin, and G. Siuzdak. “Direct characterization of solid phase resin-bound molecules by mass spectrometry.” Bioorganic and Medicinal Chemistry Letters 6, no. 8 (April 23, 1996): 979–82. https://doi.org/10.1016/0960-894X(96)00152-7.
Fitzgerald MC, Harris K, Shevlin CG, Siuzdak G. Direct characterization of solid phase resin-bound molecules by mass spectrometry. Bioorganic and Medicinal Chemistry Letters. 1996 Apr 23;6(8):979–82.
Fitzgerald, M. C., et al. “Direct characterization of solid phase resin-bound molecules by mass spectrometry.” Bioorganic and Medicinal Chemistry Letters, vol. 6, no. 8, Apr. 1996, pp. 979–82. Scopus, doi:10.1016/0960-894X(96)00152-7.
Fitzgerald MC, Harris K, Shevlin CG, Siuzdak G. Direct characterization of solid phase resin-bound molecules by mass spectrometry. Bioorganic and Medicinal Chemistry Letters. 1996 Apr 23;6(8):979–982.
Published In
Bioorganic and Medicinal Chemistry Letters
DOI
ISSN
0960-894X
Publication Date
April 23, 1996
Volume
6
Issue
8
Start / End Page
979 / 982
Related Subject Headings
- Medicinal & Biomolecular Chemistry
- 3405 Organic chemistry
- 3404 Medicinal and biomolecular chemistry
- 1115 Pharmacology and Pharmaceutical Sciences
- 0305 Organic Chemistry
- 0304 Medicinal and Biomolecular Chemistry