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Lanthanide-binding tags as versatile protein coexpression probes.

Publication ,  Journal Article
Franz, KJ; Nitz, M; Imperiali, B
Published in: Chembiochem : a European journal of chemical biology
April 2003

Comprehensive proteomic analyses require new methodologies to accelerate the correlation of gene sequence with protein function. Key tools for such efforts include biophysical probes that integrate into the covalent architecture of proteins. Lanthanide-binding tags (LBTs) are expressible, multitasking fusion partners that are optimized to bind lanthanide ions and have several desirable attributes, which include long-lived luminescence, excellent X-ray scattering power for phase determination, and magnetic properties to facilitate NMR spectroscopic structure elucidation. Herein, we present peptide sequences with a 40-fold higher affinity for Tb(3+) ions and significantly brighter luminescence intensity compared with existing peptides. Incorporation of an LBT onto ubiquitin as a prototype fusion protein allows the use of powerful protein-visualization techniques, which include rapid luminescence detection of LBT-tagged proteins in SDS-PAGE gels, as well as determination of protein concentrations in complex mixtures. The LBT strategy is a new alternative for expressing fluorescent fusion proteins by routine molecular biological techniques.

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Published In

Chembiochem : a European journal of chemical biology

DOI

EISSN

1439-7633

ISSN

1439-4227

Publication Date

April 2003

Volume

4

Issue

4

Start / End Page

265 / 271

Related Subject Headings

  • Ubiquitin
  • Terbium
  • Recombinant Fusion Proteins
  • Protein Binding
  • Peptides
  • Organic Chemistry
  • Molecular Probes
  • Luminescent Measurements
  • Gels
  • Electrophoresis, Polyacrylamide Gel
 

Citation

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Franz, K. J., Nitz, M., & Imperiali, B. (2003). Lanthanide-binding tags as versatile protein coexpression probes. Chembiochem : A European Journal of Chemical Biology, 4(4), 265–271. https://doi.org/10.1002/cbic.200390046
Franz, Katherine J., Mark Nitz, and Barbara Imperiali. “Lanthanide-binding tags as versatile protein coexpression probes.Chembiochem : A European Journal of Chemical Biology 4, no. 4 (April 2003): 265–71. https://doi.org/10.1002/cbic.200390046.
Franz KJ, Nitz M, Imperiali B. Lanthanide-binding tags as versatile protein coexpression probes. Chembiochem : a European journal of chemical biology. 2003 Apr;4(4):265–71.
Franz, Katherine J., et al. “Lanthanide-binding tags as versatile protein coexpression probes.Chembiochem : A European Journal of Chemical Biology, vol. 4, no. 4, Apr. 2003, pp. 265–71. Epmc, doi:10.1002/cbic.200390046.
Franz KJ, Nitz M, Imperiali B. Lanthanide-binding tags as versatile protein coexpression probes. Chembiochem : a European journal of chemical biology. 2003 Apr;4(4):265–271.
Journal cover image

Published In

Chembiochem : a European journal of chemical biology

DOI

EISSN

1439-7633

ISSN

1439-4227

Publication Date

April 2003

Volume

4

Issue

4

Start / End Page

265 / 271

Related Subject Headings

  • Ubiquitin
  • Terbium
  • Recombinant Fusion Proteins
  • Protein Binding
  • Peptides
  • Organic Chemistry
  • Molecular Probes
  • Luminescent Measurements
  • Gels
  • Electrophoresis, Polyacrylamide Gel