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Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding.

Publication ,  Journal Article
Liu, LL; Franz, KJ
Published in: Journal of the American Chemical Society
July 2005

By using Tb3+ as a luminescent probe, we demonstrate that the phosphorylation state of a 14-residue peptide fragment of alpha-synuclein, a protein implicated in Parkinson's Disease, dramatically affects the metal ion affinity of the peptide. Whereas the unphosphorylated peptide and its phosphoserine analogue show weak Tb3+ binding, its phosphotyrosine analogue shows tight 1:1 binding as well as 2:1 and 3:1 Tb:peptide adducts. Our data suggest that the phosphorylated amino acid must be appropriately positioned among additional ligating residues to establish this phosphorylation-dependent metal binding.

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Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

July 2005

Volume

127

Issue

27

Start / End Page

9662 / 9663

Related Subject Headings

  • alpha-Synuclein
  • Titrimetry
  • Terbium
  • Synucleins
  • Spectrometry, Fluorescence
  • Phosphotyrosine
  • Phosphoserine
  • Phosphorylation
  • Peptide Fragments
  • Nerve Tissue Proteins
 

Citation

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Liu, L. L., & Franz, K. J. (2005). Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding. Journal of the American Chemical Society, 127(27), 9662–9663. https://doi.org/10.1021/ja043247v
Liu, Lucy L., and Katherine J. Franz. “Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding.Journal of the American Chemical Society 127, no. 27 (July 2005): 9662–63. https://doi.org/10.1021/ja043247v.
Liu LL, Franz KJ. Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding. Journal of the American Chemical Society. 2005 Jul;127(27):9662–3.
Liu, Lucy L., and Katherine J. Franz. “Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding.Journal of the American Chemical Society, vol. 127, no. 27, July 2005, pp. 9662–63. Epmc, doi:10.1021/ja043247v.
Liu LL, Franz KJ. Phosphorylation of an alpha-synuclein peptide fragment enhances metal binding. Journal of the American Chemical Society. 2005 Jul;127(27):9662–9663.
Journal cover image

Published In

Journal of the American Chemical Society

DOI

EISSN

1520-5126

ISSN

0002-7863

Publication Date

July 2005

Volume

127

Issue

27

Start / End Page

9662 / 9663

Related Subject Headings

  • alpha-Synuclein
  • Titrimetry
  • Terbium
  • Synucleins
  • Spectrometry, Fluorescence
  • Phosphotyrosine
  • Phosphoserine
  • Phosphorylation
  • Peptide Fragments
  • Nerve Tissue Proteins