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Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules.

Publication ,  Journal Article
Berwin, B; Rosser, MFN; Brinker, KG; Nicchitta, CV
Published in: Traffic
May 2002

GRP94 (gp96)-associated peptides can elicit cellular immune responses, an activity thought to reflect the presence of a cell surface receptor (CD91) on antigen-presenting cells that mediates GRP94 internalization and trafficking to an amenable site for peptide transfer to major histocompatibility complex class I molecules. We report that GRP94 internalized by receptor-mediated endocytosis is trafficked to a Rab5a, CD1 and transferrin-negative, Fc receptor and major histocompatibility complex class I-positive endocytic compartment. Receptor-internalized GRP94 did not access the endoplasmic reticulum of antigen-presenting cells. To identify the site of re-presentation of GRP94-associated peptides, kinetic analyses were performed utilizing GRP94-OVA (SIINFEKL) peptide complexes, with peptide re-presentation assayed with the Kb-SIINFEKL-specific MAb, 25-D1.16. Analyses of the kinetics of re-presentation of GRP94-associated peptides, under conditions in which de novo synthesis of major histocompatibility complex class I molecules was inhibited, identified a post-endoplasmic reticulum compartment, accessed by mature major histocompatibility complex class I, as the predominant site of GRP94-associated peptide exchange onto major histocompatibility complex class I.

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Published In

Traffic

DOI

ISSN

1398-9219

Publication Date

May 2002

Volume

3

Issue

5

Start / End Page

358 / 366

Location

England

Related Subject Headings

  • Peptides
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
  • Histocompatibility Antigens Class I
  • HSP70 Heat-Shock Proteins
  • Endosomes
  • Endocytosis
  • Developmental Biology
  • Animals
 

Citation

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Berwin, B., Rosser, M. F. N., Brinker, K. G., & Nicchitta, C. V. (2002). Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules. Traffic, 3(5), 358–366. https://doi.org/10.1034/j.1600-0854.2002.30505.x
Berwin, B., M. F. N. Rosser, K. G. Brinker, and C. V. Nicchitta. “Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules.Traffic 3, no. 5 (May 2002): 358–66. https://doi.org/10.1034/j.1600-0854.2002.30505.x.
Berwin B, Rosser MFN, Brinker KG, Nicchitta CV. Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules. Traffic. 2002 May;3(5):358–66.
Berwin, B., et al. “Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules.Traffic, vol. 3, no. 5, May 2002, pp. 358–66. Pubmed, doi:10.1034/j.1600-0854.2002.30505.x.
Berwin B, Rosser MFN, Brinker KG, Nicchitta CV. Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules. Traffic. 2002 May;3(5):358–366.
Journal cover image

Published In

Traffic

DOI

ISSN

1398-9219

Publication Date

May 2002

Volume

3

Issue

5

Start / End Page

358 / 366

Location

England

Related Subject Headings

  • Peptides
  • Mice, Inbred C57BL
  • Mice
  • Membrane Proteins
  • Histocompatibility Antigens Class I
  • HSP70 Heat-Shock Proteins
  • Endosomes
  • Endocytosis
  • Developmental Biology
  • Animals