Christopher Vincent Nicchitta
Professor of Cell Biology
We use a broad array of experimental approaches - biochemistry, cell biology, genomics, and computational biology - and are focusing on several related themes. First, we are working to identify the mRNA-encoded signals used to target mRNAs to the ER as well as the cellular factors that recognize these signals. One mechanism, in which a signal in nascent secretory and membrane proteins directs mRNA recruitment to the ER, has been previously described. It is clear though that there are multiple pathways that direct mRNAs to the ER, including pathways that direct cytosolic and nucleoplasmic protein-encoding mRNAs to the ER. We are also investigating how, once localized, mRNAs are anchored to the ER membrane. In a recent study, we reported that the cohort of mRNAs encoding organelle resident proteins(e.g., nuclear envelope, ER, Golgi, lysosomes, peroxisomes) are localized tothe ER and directly anchored to components of the ER membrane. We are very interested in understanding the cis-encoded anchoring signals and the integral membrane proteins that function in mRNA anchoring to biological membrane, and lastly, how direct mRNA anchoring influences mRNA translation and mRNA stability.
In parallel efforts, we discovered that mRNA translation is under distinct regulatory control in the cytosol and ER compartments, with translation being 3-5 fold more efficient on the ER. These differences are substantial and suggest that mRNA localization to the ER may represent an important post-transcriptional gene expression mechanism. To gain insight into the mechanisms and factors responsible for the compartmental regulation of mRNA translation we are using traditional biochemical approaches (pulse-labeling, cell fractionation, immunoprecipitation, proteomics) as well as genomic approaches (ribosome footprinting, deep sequencing).
Current Appointments & Affiliations
- Professor of Cell Biology, Cell Biology, Basic Science Departments 2008
- Associate Dean of Research Training, School of Medicine, Duke University 2014
- Associate Professor of Pathology, Pathology, Clinical Science Departments 2007
- Professor of Biochemistry, Biochemistry, Basic Science Departments 2009
- Member of the Duke Cancer Institute, Duke Cancer Institute, Institutes and Centers 1993
Contact Information
- 436 Nanaline H Duke, Durham, NC 27708
- Duke Box 3709, Durham, NC 27710
-
christopher.nicchitta@duke.edu
(919) 684-8948
-
Department of Cell Biology
-
Nicchitta Lab
- Background
-
Education, Training, & Certifications
- Ph.D., University of Pennsylvania 1987
-
Previous Appointments & Affiliations
- Associate Professor of Cell Biology with Tenure, Cell Biology, Basic Science Departments 2000 - 2008
- Assistant Professor of Cell Biology, Cell Biology, Basic Science Departments 1993 - 2000
- Recognition
-
In the News
-
JAN 11, 2018 -
SEP 12, 2014 U.S. News & World Report -
SEP 11, 2014 -
SEP 8, 2014
-
- Expertise
-
Global Scholarship
-
Expertise
-
- Research
-
Selected Grants
- Mechanisms of RNA localization and translational regulation on the endoplasmic reticulum awarded by National Institutes of Health 2021 - 2026
- Cell and Molecular Biology Training Program awarded by National Institutes of Health 2021 - 2026
- Defining Novel Roles of Ubiquitin Accumulation During the Mammalian Oxidative Stress Response awarded by National Institutes of Health 2022 - 2025
- Genetic and Genomics Training Grant awarded by National Institutes of Health 2020 - 2025
- Investigating the role of RNP granules during heart regeneration in the zebrafish awarded by American Heart Association 2022 - 2025
- Translational Regulation by Desmosomes awarded by National Institutes of Health 2021 - 2023
- Mechanisms and regulation of localized mRNA translation awarded by National Institutes of Health 2020 - 2022
- Mechanisms of mRNA Anchoring and Translation Regulation on the Endoplasmic Reticulum awarded by National Institutes of Health 2012 - 2021
- Super-resolution Imaging via Multiple Modalities with the Zeiss Elyra 7 awarded by National Institutes of Health 2020 - 2021
- Mechanisms of Translational Compartmentalization in Eukaryotic Cells awarded by National Institutes of Health 2017 - 2021
- Genetics Training Grant awarded by National Institutes of Health 1979 - 2020
- Organization and Function of Cellular Structure awarded by National Institutes of Health 1975 - 2020
- Thermo Lumos Tribrid High-Resolution Accurate-Mass Tandem Mass Spectrometer awarded by National Institutes of Health 2018 - 2019
- Asymmetric RNA localization in neural stem cells during cortical development awarded by National Institutes of Health 2016 - 2018
- Eukaryotic Initiation Factor 2a Signaling in Dystonia Pathogenesis and Treatment awarded by National Institutes of Health 2016 - 2018
- Dissecting the role of the exon junction complex in embryonic corticogenesis awarded by National Institutes of Health 2016 - 2017
- Instrumentation for Quantitative Phosphoproteomics and Acetylomics awarded by National Institutes of Health 2014 - 2015
- Regulation of mRNA Partitioning to the Endoplasmic Reticulum awarded by National Institutes of Health 2007 - 2012
- Role Of Surfactant In Innate and Adaptive Immunity awarded by National Institutes of Health 2001 - 2011
- Rapidly Degraded Polypeptides in Protein Quality Control awarded by National Institutes of Health 2008 - 2009
- Mechanism of Chaperone-Mediated Tumor Rejection awarded by National Institutes of Health 2004 - 2009
- The Molecular Mechanism of GRP94 Function awarded by National Institutes of Health 1997 - 2007
- Novel mechanisms governing cellular mRNA localization awarded by National Institutes of Health 2004 - 2007
- Structure and Regulation of hsp90 Chaperones awarded by National Institutes of Health 2005
- Regulation of GRP94: Targeting Novel Cancer Therapeutics awarded by National Institutes of Health 2002 - 2005
- GRP94-Mediated Immune Responses Evoked by Cell Death awarded by National Institutes of Health 2001 - 2004
- The Molecular Mechanism Of Translocation awarded by National Institutes of Health 1994 - 2003
- Same awarded by National Institutes of Health 1999 - 2002
- The Role Of Lumenal Proteins In Protein Translocation awarded by National Institutes of Health 1994 - 1999
- The Role Of Lumenal Proteins And Protein Translocation awarded by National Institutes of Health 1997 - 1998
- The Role Of Lumenal Proteins And Protein Transiocation awarded by National Institutes of Health 1995 - 1998
- Publications & Artistic Works
-
Selected Publications
-
Academic Articles
-
Child, Jessica R., Qiang Chen, David W. Reid, Sujatha Jagannathan, and Christopher V. Nicchitta. “Recruitment of endoplasmic reticulum-targeted and cytosolic mRNAs into membrane-associated stress granules.” Rna 27, no. 10 (October 2021): 1241–56. https://doi.org/10.1261/rna.078858.121.Full Text Link to Item
-
Hannigan, Molly M., Alyson M. Hoffman, J Will Thompson, Tianli Zheng, and Christopher V. Nicchitta. “Quantitative Proteomics Links the LRRC59 Interactome to mRNA Translation on the ER Membrane.” Mol Cell Proteomics 19, no. 11 (November 2020): 1826–49. https://doi.org/10.1074/mcp.RA120.002228.Full Text Link to Item
-
Hish, A. J., G. A. Nagy, C. M. Fang, L. Kelley, C. V. Nicchitta, K. Dzirasa, and M. Zachary Rosenthal. “Acceptability and perceived effectiveness of approaches to support biomedical doctoral student wellness: One size doesn⇔t fit all.” International Journal of Doctoral Studies 15 (January 1, 2020): 653–84. https://doi.org/10.28945/4669.Full Text
-
Hish, Alexander J., Gabriela A. Nagy, Caitlin M. Fang, Lisalynn Kelley, Christopher V. Nicchitta, Kafui Dzirasa, and M Zachary Rosenthal. “Applying the Stress Process Model to Stress-Burnout and Stress-Depression Relationships in Biomedical Doctoral Students: A Cross-Sectional Pilot Study.” Cbe Life Sci Educ 18, no. 4 (December 2019): ar51. https://doi.org/10.1187/cbe.19-03-0060.Full Text Link to Item
-
Nagy, Gabriela A., Caitlin M. Fang, Alexander J. Hish, Lisalynn Kelly, Christopher V. Nicchitta, Kafui Dzirasa, and M Zachary Rosenthal. “Burnout and Mental Health Problems in Biomedical Doctoral Students.” Cbe Life Sci Educ 18, no. 2 (June 2019): ar27. https://doi.org/10.1187/cbe.18-09-0198.Full Text Link to Item
-
Hoffman, Alyson M., Qiang Chen, Tianli Zheng, and Christopher V. Nicchitta. “Heterogeneous translational landscape of the endoplasmic reticulum revealed by ribosome proximity labeling and transcriptome analysis.” J Biol Chem 294, no. 22 (May 31, 2019): 8942–58. https://doi.org/10.1074/jbc.RA119.007996.Full Text Link to Item
-
Hsu, Jack C-C, David W. Reid, Alyson M. Hoffman, Devanand Sarkar, and Christopher V. Nicchitta. “Oncoprotein AEG-1 is an endoplasmic reticulum RNA-binding protein whose interactome is enriched in organelle resident protein-encoding mRNAs.” Rna 24, no. 5 (May 2018): 688–703. https://doi.org/10.1261/rna.063313.117.Full Text Link to Item
-
Reid, David W., Rafael K. Campos, Jessica R. Child, Tianli Zheng, Kitti Wing Ki Chan, Shelton S. Bradrick, Subhash G. Vasudevan, Mariano A. Garcia-Blanco, and Christopher V. Nicchitta. “Dengue Virus Selectively Annexes Endoplasmic Reticulum-Associated Translation Machinery as a Strategy for Co-opting Host Cell Protein Synthesis.” J Virol 92, no. 7 (April 1, 2018). https://doi.org/10.1128/JVI.01766-17.Full Text Open Access Copy Link to Item
-
Garcia-Blanco, Mariano A., Subhash G. Vasudevan, Shelton S. Bradrick, and Christopher Nicchitta. “Flavivirus RNA transactions from viral entry to genome replication.” Antiviral Res 134 (October 2016): 244–49. https://doi.org/10.1016/j.antiviral.2016.09.010.Full Text Link to Item
-
Reid, David W., Angeline S. L. Tay, Jeyapriya R. Sundaram, Irene C. J. Lee, Qiang Chen, Simi E. George, Christopher V. Nicchitta, and Shirish Shenolikar. “Complementary Roles of GADD34- and CReP-Containing Eukaryotic Initiation Factor 2α Phosphatases during the Unfolded Protein Response.” Mol Cell Biol 36, no. 13 (July 1, 2016): 1868–80. https://doi.org/10.1128/MCB.00190-16.Full Text Open Access Copy Link to Item
-
LaMonte, Gregory, Katelyn A. Walzer, Joshua Lacsina, Christopher Nicchitta, and Jen-Tsan Chi. “Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum.” J Vis Exp, no. 106 (December 4, 2015): e53214. https://doi.org/10.3791/53214.Full Text Link to Item
-
Reid, David W., Shirish Shenolikar, and Christopher V. Nicchitta. “Simple and inexpensive ribosome profiling analysis of mRNA translation.” Methods 91 (December 2015): 69–74. https://doi.org/10.1016/j.ymeth.2015.07.003.Full Text Open Access Copy Link to Item
-
Reid, David W., and Christopher V. Nicchitta. “LOCAL TRANSLATION. Comment on "Principles of ER cotranslational translocation revealed by proximity-specific ribosome profiling".” Science 348, no. 6240 (June 12, 2015): 1217. https://doi.org/10.1126/science.aaa7257.Full Text Link to Item
-
Reid, David W., and Christopher V. Nicchitta. “Diversity and selectivity in mRNA translation on the endoplasmic reticulum.” Nat Rev Mol Cell Biol 16, no. 4 (April 2015): 221–31. https://doi.org/10.1038/nrm3958.Full Text Link to Item
-
Jagannathan, Sujatha, David W. Reid, Amanda H. Cox, and Christopher V. Nicchitta. “De novo translation initiation on membrane-bound ribosomes as a mechanism for localization of cytosolic protein mRNAs to the endoplasmic reticulum.” Rna 20, no. 10 (October 2014): 1489–98. https://doi.org/10.1261/rna.045526.114.Full Text Link to Item
-
Brooks, Susan S., Alissa L. Wall, Christelle Golzio, David W. Reid, Amalia Kondyles, Jason R. Willer, Christina Botti, Christopher V. Nicchitta, Nicholas Katsanis, and Erica E. Davis. “A novel ribosomopathy caused by dysfunction of RPL10 disrupts neurodevelopment and causes X-linked microcephaly in humans.” Genetics 198, no. 2 (October 2014): 723–33. https://doi.org/10.1534/genetics.114.168211.Full Text Link to Item
-
Jagannathan, Sujatha, Jack C-C Hsu, David W. Reid, Qiang Chen, Will J. Thompson, Arthur M. Moseley, and Christopher V. Nicchitta. “Multifunctional roles for the protein translocation machinery in RNA anchoring to the endoplasmic reticulum.” J Biol Chem 289, no. 37 (September 12, 2014): 25907–24. https://doi.org/10.1074/jbc.M114.580688.Full Text Link to Item
-
Reid, David W., Qiang Chen, Angeline S-L Tay, Shirish Shenolikar, and Christopher V. Nicchitta. “The unfolded protein response triggers selective mRNA release from the endoplasmic reticulum.” Cell 158, no. 6 (September 11, 2014): 1362–74. https://doi.org/10.1016/j.cell.2014.08.012.Full Text Open Access Copy Link to Item
-
Lampson, Benjamin L., Nicole L. K. Pershing, Joseph A. Prinz, Joshua R. Lacsina, William F. Marzluff, Christopher V. Nicchitta, David M. MacAlpine, and Christopher M. Counter. “Rare codons regulate KRas oncogenesis.” Curr Biol 23, no. 1 (January 7, 2013): 70–75. https://doi.org/10.1016/j.cub.2012.11.031.Full Text Link to Item
-
Epple, Laura M., Rebecca D. Dodd, Andrea L. Merz, Anjelika M. Dechkovskaia, Matthew Herring, Benjamin A. Winston, Alex M. Lencioni, et al. “Induction of the unfolded protein response drives enhanced metabolism and chemoresistance in glioma cells.” Plos One 8, no. 8 (2013): e73267. https://doi.org/10.1371/journal.pone.0073267.Full Text Link to Item
-
Huang, Qi-Quan, Renee E. Koessler, Robert Birkett, Andrea Dorfleutner, Harris Perlman, G Kenneth Haines, Christian Stehlik, Christopher V. Nicchitta, and Richard M. Pope. “Glycoprotein 96 perpetuates the persistent inflammation of rheumatoid arthritis.” Arthritis Rheum 64, no. 11 (November 2012): 3638–48. https://doi.org/10.1002/art.34610.Full Text Link to Item
-
LaMonte, Gregory, Nisha Philip, Joseph Reardon, Joshua R. Lacsina, William Majoros, Lesley Chapman, Courtney D. Thornburg, et al. “Translocation of sickle cell erythrocyte microRNAs into Plasmodium falciparum inhibits parasite translation and contributes to malaria resistance.” Cell Host Microbe 12, no. 2 (August 16, 2012): 187–99. https://doi.org/10.1016/j.chom.2012.06.007.Full Text Link to Item
-
Duerfeldt, Adam S., Laura B. Peterson, Jason C. Maynard, Chun Leung Ng, Davide Eletto, Olga Ostrovsky, Heather E. Shinogle, et al. “Development of a Grp94 inhibitor.” J Am Chem Soc 134, no. 23 (June 13, 2012): 9796–9804. https://doi.org/10.1021/ja303477g.Full Text Link to Item
-
Reid, David W., and Christopher V. Nicchitta. “The enduring enigma of nuclear translation.” J Cell Biol 197, no. 1 (April 2, 2012): 7–9. https://doi.org/10.1083/jcb.201202140.Full Text Link to Item
-
Reid, David W., and Christopher V. Nicchitta. “Primary role for endoplasmic reticulum-bound ribosomes in cellular translation identified by ribosome profiling.” J Biol Chem 287, no. 8 (February 17, 2012): 5518–27. https://doi.org/10.1074/jbc.M111.312280.Full Text Link to Item
-
Lacsina, Joshua R., Odessa A. Marks, Xiongfei Liu, David W. Reid, Sujatha Jagannathan, and Christopher V. Nicchitta. “Premature translational termination products are rapidly degraded substrates for MHC class I presentation.” Plos One 7, no. 12 (2012): e51968. https://doi.org/10.1371/journal.pone.0051968.Full Text Link to Item
-
Lacsina, Joshua R., Gregory LaMonte, Christopher V. Nicchitta, and Jen-Tsan Chi. “Polysome profiling of the malaria parasite Plasmodium falciparum.” Mol Biochem Parasitol 179, no. 1 (September 2011): 42–46. https://doi.org/10.1016/j.molbiopara.2011.05.003.Full Text Link to Item
-
Chen, Qiang, Sujatha Jagannathan, David W. Reid, Tianli Zheng, and Christopher V. Nicchitta. “Hierarchical regulation of mRNA partitioning between the cytoplasm and the endoplasmic reticulum of mammalian cells.” Mol Biol Cell 22, no. 14 (July 15, 2011): 2646–58. https://doi.org/10.1091/mbc.E11-03-0239.Full Text Link to Item
-
Yewdell, Jonathan W., Joshua R. Lacsina, Martin C. Rechsteiner, and Christopher V. Nicchitta. “Out with the old, in with the new? Comparing methods for measuring protein degradation.” Cell Biol Int 35, no. 5 (May 2011): 457–62. https://doi.org/10.1042/CBI20110055.Full Text Link to Item
-
Jagannathan, Sujatha, Christine Nwosu, and Christopher V. Nicchitta. “Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation.” Methods Mol Biol 714 (2011): 301–21. https://doi.org/10.1007/978-1-61779-005-8_19.Full Text Link to Item
-
Jockheck-Clark, Angela R., Edith V. Bowers, Mariam B. Totonchy, Julie Neubauer, Salvatore V. Pizzo, and Christopher V. Nicchitta. “Re-examination of CD91 function in GRP94 (glycoprotein 96) surface binding, uptake, and peptide cross-presentation.” J Immunol 185, no. 11 (December 1, 2010): 6819–30. https://doi.org/10.4049/jimmunol.1000448.Full Text Link to Item
-
Maynard, Jason C., Trang Pham, Tianli Zheng, Angela Jockheck-Clark, Helen B. Rankin, Christopher B. Newgard, Eric P. Spana, and Christopher V. Nicchitta. “Gp93, the Drosophila GRP94 ortholog, is required for gut epithelial homeostasis and nutrient assimilation-coupled growth control.” Dev Biol 339, no. 2 (March 15, 2010): 295–306. https://doi.org/10.1016/j.ydbio.2009.12.023.Full Text Link to Item
-
Lu, Zheming, Lei Zhou, Patrick Killela, Ahmed B. Rasheed, Chunhui Di, William E. Poe, Roger E. McLendon, Darell D. Bigner, Christopher Nicchitta, and Hai Yan. “Glioblastoma proto-oncogene SEC61gamma is required for tumor cell survival and response to endoplasmic reticulum stress.” Cancer Res 69, no. 23 (December 1, 2009): 9105–11. https://doi.org/10.1158/0008-5472.CAN-09-2775.Full Text Link to Item
-
Lev, A., P. Dimberu, S. R. Das, J. C. Maynard, C. V. Nicchitta, J. R. Bennink, and J. W. Yewdell. “Efficient cross-priming of antiviral CD8+ T cells by antigen donor cells is GRP94 independent (Journal of Immunology (2009) 183, (4205-4210)).” Journal of Immunology 183, no. 7 (October 1, 2009). https://doi.org/10.4049/jimmunol.1090046.Full Text
-
Lev, Avital, Peniel Dimberu, Suman R. Das, Jason C. Maynard, Christopher V. Nicchitta, Jack R. Bennink, and Jonathan W. Yewdell. “Efficient cross-priming of antiviral CD8+ T cells by antigen donor cells is GRP94 independent.” J Immunol 183, no. 7 (October 1, 2009): 4205–10. https://doi.org/10.4049/jimmunol.0901828.Full Text Link to Item
-
Huang, Qi-Quan, Rudina Sobkoviak, Angela R. Jockheck-Clark, Bo Shi, Arthur M. Mandelin, Paul Peter Tak, G Kennith Haines, Christopher V. Nicchitta, and Richard M. Pope. “Heat shock protein 96 is elevated in rheumatoid arthritis and activates macrophages primarily via TLR2 signaling.” J Immunol 182, no. 8 (April 15, 2009): 4965–73. https://doi.org/10.4049/jimmunol.0801563.Full Text Link to Item
-
Nicchitta, Christopher V. “Cell biology: How to combat stress.” Nature 457, no. 7230 (February 5, 2009): 668–69. https://doi.org/10.1038/457668a.Full Text Link to Item
-
Tewalt, Eric F., Jason C. Maynard, Julie Jo Walters, Amanda M. Schell, Brent L. Berwin, Christopher V. Nicchitta, and Christopher C. Norbury. “Redundancy renders the glycoprotein 96 receptor scavenger receptor A dispensable for cross priming in vivo.” Immunology 125, no. 4 (December 2008): 480–91. https://doi.org/10.1111/j.1365-2567.2008.02861.x.Full Text Link to Item
-
Lev, Avital, Kazuyo Takeda, Damien Zanker, Jason C. Maynard, Peniel Dimberu, Elizabeth Waffarn, James Gibbs, et al. “The exception that reinforces the rule: crosspriming by cytosolic peptides that escape degradation.” Immunity 28, no. 6 (June 2008): 787–98. https://doi.org/10.1016/j.immuni.2008.04.015.Full Text Link to Item
-
Pyhtila, Brook, Tianli Zheng, Patrick J. Lager, Jack D. Keene, Mary C. Reedy, and Christopher V. Nicchitta. “Signal sequence- and translation-independent mRNA localization to the endoplasmic reticulum.” Rna 14, no. 3 (March 2008): 445–53. https://doi.org/10.1261/rna.721108.Full Text Link to Item
-
Stephens, Samuel B., and Christopher V. Nicchitta. “Divergent regulation of protein synthesis in the cytosol and endoplasmic reticulum compartments of mammalian cells.” Mol Biol Cell 19, no. 2 (February 2008): 623–32. https://doi.org/10.1091/mbc.e07-07-0677.Full Text Link to Item
-
Stephens, Samuel B., Rebecca D. Dodd, Rachel S. Lerner, Brook M. Pyhtila, and Christopher V. Nicchitta. “Analysis of mRNA partitioning between the cytosol and endoplasmic reticulum compartments of mammalian cells.” Methods Mol Biol 419 (2008): 197–214. https://doi.org/10.1007/978-1-59745-033-1_14.Full Text Link to Item
-
Stephens, Samuel B., and Christopher V. Nicchitta. “In vitro and tissue culture methods for analysis of translation initiation on the endoplasmic reticulum.” Methods Enzymol 431 (2007): 47–60. https://doi.org/10.1016/S0076-6879(07)31004-5.Full Text Link to Item
-
Warger, Tobias, Nobert Hilf, Gerd Rechtsteiner, Philipp Haselmayer, Deanna M. Carrick, Helmut Jonuleit, Philipp von Landenberg, et al. “Interaction of TLR2 and TLR4 ligands with the N-terminal domain of Gp96 amplifies innate and adaptive immune responses.” J Biol Chem 281, no. 32 (August 11, 2006): 22545–53. https://doi.org/10.1074/jbc.M502900200.Full Text Link to Item
-
Yewdell, Jonathan W., and Christopher V. Nicchitta. “The DRiP hypothesis decennial: support, controversy, refinement and extension.” Trends Immunol 27, no. 8 (August 2006): 368–73. https://doi.org/10.1016/j.it.2006.06.008.Full Text Link to Item
-
Chiosis, Gabriela, Julia Aguirre, and Christopher V. Nicchitta. “Synthesis of Hsp90 dimerization modulators.” Bioorg Med Chem Lett 16, no. 13 (July 1, 2006): 3529–32. https://doi.org/10.1016/j.bmcl.2006.03.092.Full Text Link to Item
-
Chu, Feixia, Jason C. Maynard, Gabriela Chiosis, Christopher V. Nicchitta, and Alma L. Burlingame. “Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94.” Protein Sci 15, no. 6 (June 2006): 1260–69. https://doi.org/10.1110/ps.052065106.Full Text Link to Item
-
Lerner, Rachel S., and Christopher V. Nicchitta. “mRNA translation is compartmentalized to the endoplasmic reticulum following physiological inhibition of cap-dependent translation.” Rna 12, no. 5 (May 2006): 775–89. https://doi.org/10.1261/rna.2318906.Full Text Link to Item
-
Nicchitta, Christopher V., Rachel S. Lerner, Samuel B. Stephens, Rebecca D. Dodd, and Brook Pyhtila. “Pathways for compartmentalizing protein synthesis in eukaryotic cells: the template-partitioning model.” Biochem Cell Biol 83, no. 6 (December 2005): 687–95. https://doi.org/10.1139/o05-147.Full Text Link to Item
-
Stephens, Samuel B., Rebecca D. Dodd, Joseph W. Brewer, Patrick J. Lager, Jack D. Keene, and Christopher V. Nicchitta. “Stable ribosome binding to the endoplasmic reticulum enables compartment-specific regulation of mRNA translation.” Mol Biol Cell 16, no. 12 (December 2005): 5819–31. https://doi.org/10.1091/mbc.e05-07-0685.Full Text Link to Item
-
Liu, Shanling, He Wang, Zhonghui Yang, Takashi Kon, Jiangao Zhu, Yiting Cao, Fang Li, John Kirkpatrick, Christopher V. Nicchitta, and Chuan-Yuan Li. “Enhancement of cancer radiation therapy by use of adenovirus-mediated secretable glucose-regulated protein 94/gp96 expression.” Cancer Res 65, no. 20 (October 15, 2005): 9126–31. https://doi.org/10.1158/0008-5472.CAN-05-0945.Full Text Link to Item
-
Kovacs, Jeffrey J., Patrick J. M. Murphy, Stéphanie Gaillard, Xuan Zhao, June-Tai Wu, Christopher V. Nicchitta, Minoru Yoshida, David O. Toft, William B. Pratt, and Tso-Pang Yao. “HDAC6 regulates Hsp90 acetylation and chaperone-dependent activation of glucocorticoid receptor.” Mol Cell 18, no. 5 (May 27, 2005): 601–7. https://doi.org/10.1016/j.molcel.2005.04.021.Full Text Link to Item
-
Parsa, Cyrus J., Robyn C. Reed, G Brant Walton, Laura S. Pascal, Richard B. Thompson, Jason A. Petrofski, Sitaram M. Emani, et al. “Catheter-mediated subselective intracoronary gene delivery to the rabbit heart: introduction of a novel method.” J Gene Med 7, no. 5 (May 2005): 595–603. https://doi.org/10.1002/jgm.704.Full Text Link to Item
-
Tie, Jian-Ke, Christopher Nicchitta, Gunnar von Heijne, and Darrel W. Stafford. “Membrane topology mapping of vitamin K epoxide reductase by in vitro translation/cotranslocation.” J Biol Chem 280, no. 16 (April 22, 2005): 16410–16. https://doi.org/10.1074/jbc.M500765200.Full Text Link to Item
-
Rosser, Meredith F. N., Brian M. Trotta, Megan R. Marshall, Brent Berwin, and Christopher V. Nicchitta. “Adenosine nucleotides and the regulation of GRP94-client protein interactions.” Biochemistry 43, no. 27 (July 13, 2004): 8835–45. https://doi.org/10.1021/bi049539q.Full Text Link to Item
-
Baker-LePain, Julie C., Marcella Sarzotti, and Christopher V. Nicchitta. “Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo.” J Immunol 172, no. 7 (April 1, 2004): 4195–4203. https://doi.org/10.4049/jimmunol.172.7.4195.Full Text Link to Item
-
Nicchitta, Christopher V., Deanna M. Carrick, and Julie C. Baker-Lepain. “The messenger and the message: gp96 (GRP94)-peptide interactions in cellular immunity.” Cell Stress Chaperones 9, no. 4 (2004): 325–31. https://doi.org/10.1379/csc-62.1.Full Text Link to Item
-
Soldano, Karen L., Arif Jivan, Christopher V. Nicchitta, and Daniel T. Gewirth. “Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation.” J Biol Chem 278, no. 48 (November 28, 2003): 48330–38. https://doi.org/10.1074/jbc.M308661200.Full Text Link to Item
-
Berwin, Brent, Justin P. Hart, Stuart Rice, Cecilia Gass, Salvatore V. Pizzo, Steven R. Post, and Christopher V. Nicchitta. “Scavenger receptor-A mediates gp96/GRP94 and calreticulin internalization by antigen-presenting cells.” Embo J 22, no. 22 (November 17, 2003): 6127–36. https://doi.org/10.1093/emboj/cdg572.Full Text Link to Item
-
Lerner, Rachel S., Robert M. Seiser, Tianli Zheng, Patrick J. Lager, Mary C. Reedy, Jack D. Keene, and Christopher V. Nicchitta. “Partitioning and translation of mRNAs encoding soluble proteins on membrane-bound ribosomes.” Rna 9, no. 9 (September 2003): 1123–37. https://doi.org/10.1261/rna.5610403.Full Text Link to Item
-
Nicchitta, Christopher V. “Come forth CD1d: Hsp110 in the regulation of intestinal epithelial CD1d expression.” J Clin Invest 112, no. 5 (September 2003): 646–48. https://doi.org/10.1172/JCI19641.Full Text Link to Item
-
Reed, Robyn C., Brent Berwin, Jeffrey P. Baker, and Christopher V. Nicchitta. “GRP94/gp96 elicits ERK activation in murine macrophages. A role for endotoxin contamination in NF-kappa B activation and nitric oxide production.” J Biol Chem 278, no. 34 (August 22, 2003): 31853–60. https://doi.org/10.1074/jbc.M305480200.Full Text Link to Item
-
Nicchitta, Christopher V. “Re-evaluating the role of heat-shock protein-peptide interactions in tumour immunity.” Nat Rev Immunol 3, no. 5 (May 2003): 427–32. https://doi.org/10.1038/nri1089.Full Text Link to Item
-
Baker-LePain, Julie C., Robyn C. Reed, and Christopher V. Nicchitta. “ISO: a critical evaluation of the role of peptides in heat shock/chaperone protein-mediated tumor rejection.” Curr Opin Immunol 15, no. 1 (February 2003): 89–94. https://doi.org/10.1016/s0952791502000067.Full Text Link to Item
-
Baker-LePain, Julie C., Marcella Sarzotti, Timothy A. Fields, Chuan-Yuan Li, and Christopher V. Nicchitta. “GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.” J Exp Med 196, no. 11 (December 2, 2002): 1447–59. https://doi.org/10.1084/jem.20020436.Full Text Link to Item
-
Chu, F., P. Baker, S. Mahrus, C. S. Craik, M. Rosser, C. V. Nicchitta, and A. L. Burlingame. “Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS.” Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 289–90.
-
Nicchitta, Christopher V. “A platform for compartmentalized protein synthesis: protein translation and translocation in the ER.” Curr Opin Cell Biol 14, no. 4 (August 2002): 412–16. https://doi.org/10.1016/s0955-0674(02)00353-8.Full Text Link to Item
-
Reed, Robyn C., Tianli Zheng, and Christopher V. Nicchitta. “GRP94-associated enzymatic activities. Resolution by chromatographic fractionation.” J Biol Chem 277, no. 28 (July 12, 2002): 25082–89. https://doi.org/10.1074/jbc.M203195200.Full Text Link to Item
-
Potter, Matthew D., and Christopher V. Nicchitta. “Endoplasmic reticulum-bound ribosomes reside in stable association with the translocon following termination of protein synthesis.” J Biol Chem 277, no. 26 (June 28, 2002): 23314–20. https://doi.org/10.1074/jbc.M202559200.Full Text Link to Item
-
Berwin, B., M. F. N. Rosser, K. G. Brinker, and C. V. Nicchitta. “Transfer of GRP94(Gp96)-associated peptides onto endosomal MHC class I molecules.” Traffic 3, no. 5 (May 2002): 358–66. https://doi.org/10.1034/j.1600-0854.2002.30505.x.Full Text Link to Item
-
Berwin, Brent, Justin P. Hart, Salvatore V. Pizzo, and Christopher V. Nicchitta. “Cutting edge: CD91-independent cross-presentation of GRP94(gp96)-associated peptides.” J Immunol 168, no. 9 (May 1, 2002): 4282–86. https://doi.org/10.4049/jimmunol.168.9.4282.Full Text Link to Item
-
Nicchitta, C. V. “Signal sequence function in the mammalian endoplasmic reticulum: A biological perspective.” Current Topics in Membranes 52 (January 1, 2002): 483–99. https://doi.org/10.1016/s1063-5823(02)52019-6.Full Text
-
Berwin, B., and C. V. Nicchitta. “To find the road traveled to tumor immunity: the trafficking itineraries of molecular chaperones in antigen-presenting cells.” Traffic 2, no. 10 (October 2001): 690–97. https://doi.org/10.1034/j.1600-0854.2001.21003.x.Full Text Link to Item
-
Berwin, B., R. C. Reed, and C. V. Nicchitta. “Virally induced lytic cell death elicits the release of immunogenic GRP94/gp96.” J Biol Chem 276, no. 24 (June 15, 2001): 21083–88. https://doi.org/10.1074/jbc.M101836200.Full Text Link to Item
-
Potter, M. D., R. M. Seiser, and C. V. Nicchitta. “Ribosome exchange revisited: a mechanism for translation-coupled ribosome detachment from the ER membrane.” Trends Cell Biol 11, no. 3 (March 2001): 112–15. https://doi.org/10.1016/s0962-8924(00)01905-x.Full Text Link to Item
-
Xu, W., E. Mimnaugh, M. F. Rosser, C. Nicchitta, M. Marcu, Y. Yarden, and L. Neckers. “Sensitivity of mature Erbb2 to geldanamycin is conferred by its kinase domain and is mediated by the chaperone protein Hsp90.” J Biol Chem 276, no. 5 (February 2, 2001): 3702–8. https://doi.org/10.1074/jbc.M006864200.Full Text Link to Item
-
Potter, M. D., and C. V. Nicchitta. “Regulation of ribosome detachment from the mammalian endoplasmic reticulum membrane.” J Biol Chem 275, no. 43 (October 27, 2000): 33828–35. https://doi.org/10.1074/jbc.M005294200.Full Text Link to Item
-
Seiser, R. M., and C. V. Nicchitta. “The fate of membrane-bound ribosomes following the termination of protein synthesis.” J Biol Chem 275, no. 43 (October 27, 2000): 33820–27. https://doi.org/10.1074/jbc.M004462200.Full Text Link to Item
-
Reed, R. C., and C. V. Nicchitta. “Chaperone-mediated cross-priming: a hitchhiker's guide to vesicle transport (review).” Int J Mol Med 6, no. 3 (September 2000): 259–64. https://doi.org/10.3892/ijmm.6.3.259.Full Text Link to Item
-
Tie, J., S. M. Wu, D. Jin, C. V. Nicchitta, and D. W. Stafford. “A topological study of the human gamma-glutamyl carboxylase.” Blood 96, no. 3 (August 1, 2000): 973–78.Link to Item
-
Rosser, M. F., and C. V. Nicchitta. “Ligand interactions in the adenosine nucleotide-binding domain of the Hsp90 chaperone, GRP94. I. Evidence for allosteric regulation of ligand binding.” J Biol Chem 275, no. 30 (July 28, 2000): 22798–805. https://doi.org/10.1074/jbc.M001477200.Full Text Link to Item
-
Wassenberg, J. J., R. C. Reed, and C. V. Nicchitta. “Ligand interactions in the adenosine nucleotide-binding domain of the Hsp90 chaperone, GRP94. II. Ligand-mediated activation of GRP94 molecular chaperone and peptide binding activity.” J Biol Chem 275, no. 30 (July 28, 2000): 22806–14. https://doi.org/10.1074/jbc.M001476200.Full Text Link to Item
-
Nicchitta, C. V. “Role of chaperones in antigen processing.” Immunol Invest 29, no. 2 (May 2000): 101–4. https://doi.org/10.3109/08820130009062290.Full Text Link to Item
-
Potter, M. D., and C. V. Nicchitta. “Ribosome-independent regulation of translocon composition and Sec61alpha conformation.” J Biol Chem 275, no. 3 (January 21, 2000): 2037–45. https://doi.org/10.1074/jbc.275.3.2037.Full Text Link to Item
-
Nicchitta, C. V., and R. C. Reed. “The immunological properties of endoplasmic reticulum chaperones: a conflict of interest?” Essays Biochem 36 (2000): 15–25. https://doi.org/10.1042/bse0360015.Full Text Link to Item
-
Zheng, T., and C. V. Nicchitta. “Structural determinants for signal sequence function in the mammalian endoplasmic reticulum.” J Biol Chem 274, no. 51 (December 17, 1999): 36623–30. https://doi.org/10.1074/jbc.274.51.36623.Full Text Link to Item
-
Wassenberg, J. J., C. Dezfulian, and C. V. Nicchitta. “Receptor mediated and fluid phase pathways for internalization of the ER Hsp90 chaperone GRP94 in murine macrophages.” J Cell Sci 112 ( Pt 13) (July 1999): 2167–75. https://doi.org/10.1242/jcs.112.13.2167.Full Text Link to Item
-
Nair, S., P. A. Wearsch, D. A. Mitchell, J. J. Wassenberg, E. Gilboa, and C. V. Nicchitta. “Calreticulin displays in vivo peptide-binding activity and can elicit CTL responses against bound peptides.” J Immunol 162, no. 11 (June 1, 1999): 6426–32.Link to Item
-
Nicchitta, C. V. “Protein translocation across the mammalian endoplasmic reticulum: A tale of tunnels and ratchets?” Chemtracts 11, no. 6 (June 1, 1998): 408–22.
-
Wearsch, P. A., L. Voglino, and C. V. Nicchitta. “Structural transitions accompanying the activation of peptide binding to the endoplasmic reticulum Hsp90 chaperone GRP94.” Biochemistry 37, no. 16 (April 21, 1998): 5709–19. https://doi.org/10.1021/bi9801006.Full Text Link to Item
-
Nicchitta, C. V. “Biochemical, cell biological and immunological issues surrounding the endoplasmic reticulum chaperone GRP94/gp96.” Curr Opin Immunol 10, no. 1 (February 1998): 103–9. https://doi.org/10.1016/s0952-7915(98)80039-3.Full Text Link to Item
-
Nicchitta, C. V., and T. Zheng. “Regulation of the ribosome-membrane junction at early stages of presecretory protein translocation in the mammalian endoplasmic reticulum.” J Cell Biol 139, no. 7 (December 29, 1997): 1697–1708. https://doi.org/10.1083/jcb.139.7.1697.Full Text Link to Item
-
Haynes, R. L., T. Zheng, and C. V. Nicchitta. “Structure and folding of nascent polypeptide chains during protein translocation in the endoplasmic reticulum.” J Biol Chem 272, no. 27 (July 4, 1997): 17126–33. https://doi.org/10.1074/jbc.272.27.17126.Full Text Link to Item
-
Murphy, E. C., T. Zheng, and C. V. Nicchitta. “Identification of a novel stage of ribosome/nascent chain association with the endoplasmic reticulum membrane.” J Cell Biol 136, no. 6 (March 24, 1997): 1213–26. https://doi.org/10.1083/jcb.136.6.1213.Full Text Link to Item
-
Wearsch, P. A., and C. V. Nicchitta. “Interaction of endoplasmic reticulum chaperone GRP94 with peptide substrates is adenine nucleotide-independent.” J Biol Chem 272, no. 8 (February 21, 1997): 5152–56. https://doi.org/10.1074/jbc.272.8.5152.Full Text Link to Item
-
Joseph, S. K., D. Boehning, S. Pierson, and C. V. Nicchitta. “Membrane insertion, glycosylation, and oligomerization of inositol trisphosphate receptors in a cell-free translation system.” J Biol Chem 272, no. 3 (January 17, 1997): 1579–88. https://doi.org/10.1074/jbc.272.3.1579.Full Text Link to Item
-
Wearsch, P. A., and C. V. Nicchitta. “Endoplasmic reticulum chaperone GRP94 subunit assembly is regulated through a defined oligomerization domain.” Biochemistry 35, no. 51 (December 24, 1996): 16760–69. https://doi.org/10.1021/bi962068q.Full Text Link to Item
-
Rudner, X. L., C. Nicchitta, and J. S. Almenoff. “Biogenesis, cellular localization, and functional activation of the heat-stable enterotoxin receptor (guanylyl cyclase C).” Biochemistry 35, no. 33 (August 20, 1996): 10680–86. https://doi.org/10.1021/bi9607721.Full Text Link to Item
-
Wearsch, P. A., and C. V. Nicchitta. “Purification and partial molecular characterization of GRP94, an ER resident chaperone.” Protein Expr Purif 7, no. 1 (February 1996): 114–21. https://doi.org/10.1006/prep.1996.0015.Full Text Link to Item
-
Nicchitta, C. V. “Protein translocation in the endoplasmic reticulum: The search for a unified molecular mechanism.” Seminars in Cell and Developmental Biology 7, no. 4 (January 1, 1996): 497–503. https://doi.org/10.1006/scdb.1996.0062.Full Text
-
Nicchitta, C. V., E. C. Murphy, R. Haynes, and G. S. Shelness. “Stage- and ribosome-specific alterations in nascent chain-Sec61p interactions accompany translocation across the ER membrane.” J Cell Biol 129, no. 4 (May 1995): 957–70. https://doi.org/10.1083/jcb.129.4.957.Full Text Link to Item
-
Yu, H., C. V. Nicchitta, J. Kumar, M. Becker, I. Toyoshima, and M. P. Sheetz. “Characterization of kinectin, a kinesin-binding protein: primary sequence and N-terminal topogenic signal analysis.” Mol Biol Cell 6, no. 2 (February 1995): 171–83. https://doi.org/10.1091/mbc.6.2.171.Full Text Link to Item
-
Nicchitta, C. V., and G. Blobel. “Lumenal proteins of the mammalian endoplasmic reticulum are required to complete protein translocation.” Cell 73, no. 5 (June 4, 1993): 989–98. https://doi.org/10.1016/0092-8674(93)90276-v.Full Text Link to Item
-
Shelness, G. S., L. Lin, and C. V. Nicchitta. “Membrane topology and biogenesis of eukaryotic signal peptidase.” J Biol Chem 268, no. 7 (March 5, 1993): 5201–8.Link to Item
-
Migliaccio, G., C. V. Nicchitta, and G. Blobel. “The signal sequence receptor, unlike the signal recognition particle receptor, is not essential for protein translocation.” J Cell Biol 117, no. 1 (April 1992): 15–25. https://doi.org/10.1083/jcb.117.1.15.Full Text Link to Item
-
Nicchitta, C. V., G. Migliaccio, and G. Blobel. “Biochemical fractionation and assembly of the membrane components that mediate nascent chain targeting and translocation.” Cell 65, no. 4 (May 17, 1991): 587–98. https://doi.org/10.1016/0092-8674(91)90091-c.Full Text Link to Item
-
Nicchitta, C., G. Migliaccio, and G. Blobel. “Reconstitution of secretory protein translocation from detergent-solubilized rough microsomes.” Methods Cell Biol 34 (1991): 263–85. https://doi.org/10.1016/s0091-679x(08)61685-4.Full Text Link to Item
-
Watanabe, M., C. V. Nicchitta, and G. Blobel. “Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.” Proc Natl Acad Sci U S A 87, no. 5 (March 1990): 1960–64. https://doi.org/10.1073/pnas.87.5.1960.Full Text Link to Item
-
Nicchitta, C. V., and G. Blobel. “Assembly of translocation-competent proteoliposomes from detergent-solubilized rough microsomes.” Cell 60, no. 2 (January 26, 1990): 259–69. https://doi.org/10.1016/0092-8674(90)90741-v.Full Text Link to Item
-
Nicchitta, C. V., and G. Blobel. “Nascent secretory chain binding and translocation are distinct processes: differentiation by chemical alkylation.” J Cell Biol 108, no. 3 (March 1989): 789–95. https://doi.org/10.1083/jcb.108.3.789.Full Text Link to Item
-
Joseph, S. K., H. L. Rice, and C. V. Nicchitta. “Characteristics of GTP-mediated microsomal Ca2+ release.” Biochim Biophys Acta 945, no. 2 (November 22, 1988): 185–94. https://doi.org/10.1016/0005-2736(88)90481-6.Full Text Link to Item
-
Nicchitta, C. V., S. K. Joseph, and J. R. Williamson. “GTP-mediated Ca2+ release in rough endoplasmic reticulum. Correlation with a GTP-sensitive increase in membrane permeability.” Biochem J 248, no. 3 (December 15, 1987): 741–47. https://doi.org/10.1042/bj2480741.Full Text Link to Item
-
Nicchitta, C. V., S. K. Joseph, and J. R. Williamson. “Polyethylene glycol-stimulated microsomal GTP hydrolysis. Relationship to GTP-mediated Ca2+ release.” Journal of Pharmacology and Experimental Therapeutics 240, no. 1 (January 1, 1987): 243–48.
-
Nicchitta, C. V., S. K. Joseph, and J. R. Williamson. “Polyethylene glycol-stimulated microsomal GTP hydrolysis. Relationship to GTP-mediated Ca2+ release.” Febs Lett 209, no. 2 (December 15, 1986): 243–48. https://doi.org/10.1016/0014-5793(86)81120-6.Full Text Link to Item
-
Nicchitta, C. V., M. Kamoun, and J. R. Williamson. “Cyclosporine augments receptor-mediated cellular Ca2+ fluxes in isolated hepatocytes.” J Biol Chem 260, no. 25 (November 5, 1985): 13613–18.Link to Item
-
Nicchitta, C. V., and J. R. Williamson. “Spermine. A regulator of mitochondrial calcium cycling.” J Biol Chem 259, no. 21 (November 10, 1984): 12978–83.Link to Item
-
Fiore, G. B., C. V. Nicchitta, and W. R. Ellington. “High-performance liquid chromatographic separation and quantification of alanopine and strombine in crude tissue extracts.” Anal Biochem 139, no. 2 (June 1984): 413–17. https://doi.org/10.1016/0003-2697(84)90027-7.Full Text Link to Item
-
Nicchitta, C. V., and J. R. Williamson. “Effects of spermine on Ca2+-cycling in rat liver mitochondria.” Federation Proceedings 43, no. 6 (January 1, 1984).
-
Nicchitta, C. V., and W. R. Ellington. “Partial purification and characterization of a strombine dehydrogenase from the adductor muscle of the mussel Modiolus squamosus.” Comparative Biochemistry and Physiology Part B: Biochemistry And 77, no. 1 (January 1, 1984): 233–36. https://doi.org/10.1016/0305-0491(84)90249-9.Full Text
-
-
- Teaching & Mentoring
-
Recent Courses
- BIOCHEM 393: Research Independent Study 2022
- BIOCHEM 593: Research Independent Study 2022
- CELLBIO 493: Research Independent Study 2022
- CMB 710A: Cell & Molecular Biology Module I 2022
- BIOCHEM 393: Research Independent Study 2021
- BIOCHEM 593: Research Independent Study 2021
- CELLBIO 493: Research Independent Study 2021
- CMB 710A: Cell & Molecular Biology Module I 2021
- UPGEN 701: Advanced Topics in Genetics and Genomics 2021
- BIOCHEM 393: Research Independent Study 2020
- BIOCHEM 593: Research Independent Study 2020
- CELLBIO 493: Research Independent Study 2020
- CMB 710B: Cell & Molecular Biology Module II 2020
- PATHOL 293: Research Independent Study 2020
- PATHOL 793: Research Independent Study 2020
Some information on this profile has been compiled automatically from Duke databases and external sources. (Our About page explains how this works.) If you see a problem with the information, please write to Scholars@Duke and let us know. We will reply promptly.